Cited 21 times in
Comparison of allergenic components between German cockorach whole body and fecal extract
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박중원 | - |
dc.date.accessioned | 2016-02-19T11:13:26Z | - |
dc.date.available | 2016-02-19T11:13:26Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 1081-1206 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/142670 | - |
dc.description.abstract | BACKGROUND: Cockroaches have been demonstrated to be an etiologic factor in allergic diseases. Further, sensitivity to cockroach places patients with asthma at risk for exacerbations that require emergency medical care. OBJECTIVE: This study compared the differences in allergenic components between German cockroach whole body and German cockroach fecal extracts (GWBE and GFE). METHODS: Patients with asthma and/or allergic rhinitis were skin prick tested with German cockroach extract (Bayer Corporation, West Haven, CT). Serum specimens from these patients, 25 with positive skin tests and 8 with negative tests, were used for the ELISA and immunoblot experiments. RESULTS: By ELISA, 72% (18 of 25) and 60% (15 of 25) of positive responders' sera showed IgE antibodies to GWBE and GFE, respectively, and the IgE levels to GWBE were highly correlated with those to GFE (r = .84, P < .01). In inhibition ELISA experiments, extensive cross-reactivity was observed between GWBE and GFE, slight cross-reactivity between GWBE and Dermatophagoides farinae, and no cross-reactivity between GFE and D. farinae. The two-site monoclonal antibody ELISA detected more of the German cockroach major allergens in GFE compared with GWBE; 6.2 times (2420 vs 390 U/mL) for Bla g 1 and 3 times (15.32 vs 5.07 microg/mL) for Bla g 2. In the immunoblot comparison of patients' sera, the IgE antibodies binding to GWBE were apparently different from those binding to GFE in all the positive responders' sera; eg, 50% or more of the 25 positive responders' sera reacted to 43- to 67-kDa proteins in GWBE and to 28- to 30-kDa proteins in GFE, respectively. No IgE antibodies bound to components in GWBE and GFE in the 8 negative responders' sera. CONCLUSIONS: There are major differences between the allergenic components of GWBE and GFE. Based on the amounts of major allergens (Bla g 1, Bla g 2), German cockroach feces are a more important source of allergen than the whole body in respiratory allergic diseases. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 551~556 | - |
dc.relation.isPartOf | ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Allergens/immunology | - |
dc.subject.MESH | Allergens/isolation & purification* | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antibodies, Monoclonal/immunology | - |
dc.subject.MESH | Antibody Specificity | - |
dc.subject.MESH | Antigens, Plant | - |
dc.subject.MESH | Aspartic Acid Endopeptidases/immunology | - |
dc.subject.MESH | Aspartic Acid Endopeptidases/isolation & purification | - |
dc.subject.MESH | Asthma/blood | - |
dc.subject.MESH | Asthma/immunology | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Cockroaches/chemistry | - |
dc.subject.MESH | Cockroaches/immunology* | - |
dc.subject.MESH | Cross Reactions | - |
dc.subject.MESH | Electrophoresis, Polyacrylamide Gel | - |
dc.subject.MESH | Enzyme-Linked Immunosorbent Assay | - |
dc.subject.MESH | Feces/chemistry* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunoglobulin E/immunology | - |
dc.subject.MESH | Insect Proteins/immunology | - |
dc.subject.MESH | Insect Proteins/isolation & purification* | - |
dc.subject.MESH | Mites/immunology | - |
dc.subject.MESH | Rhinitis, Allergic, Perennial/blood | - |
dc.subject.MESH | Rhinitis, Allergic, Perennial/immunology | - |
dc.subject.MESH | Skin Tests | - |
dc.subject.MESH | Tissue Extracts/analysis | - |
dc.subject.MESH | Tissue Extracts/immunology | - |
dc.title | Comparison of allergenic components between German cockorach whole body and fecal extract | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Yeong-Yeon Yun | - |
dc.contributor.googleauthor | Si H. Ko | - |
dc.contributor.googleauthor | Jung Won Park | - |
dc.contributor.googleauthor | In-Yong Lee | - |
dc.contributor.googleauthor | Han Il Ree | - |
dc.contributor.googleauthor | Chein-Soo Hong | - |
dc.identifier.doi | 10.1016/S1081-1206(10)62904-3 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01681 | - |
dc.relation.journalcode | J00153 | - |
dc.identifier.eissn | 1534-4436 | - |
dc.identifier.pmid | 11379807 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S1081120610629043?np=y | - |
dc.contributor.alternativeName | Park, Jung Won | - |
dc.contributor.affiliatedAuthor | Park, Jung Won | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 86 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 551 | - |
dc.citation.endPage | 556 | - |
dc.identifier.bibliographicCitation | ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY, Vol.86(5) : 551-556, 2001 | - |
dc.identifier.rimsid | 29756 | - |
dc.type.rims | ART | - |
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