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Effect of bradykinin on cultured bovine corneal endothelial cells

Authors
 Yang S.W. ; Lee W.K. ; Hahn T.W. ; Rha H.K. ; Lee K.H. ; Lim Y. ; Kim K.Y. ; Lee E.J. 
Citation
 Ophthalmologica, Vol.215(4) : 303~308, 2001 
Journal Title
 Ophthalmologica 
ISSN
 0030-3755 
Issue Date
2001
Abstract
PURPOSE: To clarify the effect of bradykinin on cytosolic free calcium mobilization and cell proliferation in cultured bovine corneal endothelial cells (BCEC). METHODS: The cytosolic free calcium concentration (Ca2+]i) was measured with the InCa(TM) Imaging System after the treatment of bradykinin (10(-11) to 10(-7) M) alone or with the pretreatments of EGTA, bradykinin receptor (Bk1 and Bk2) antagonists and an inhibition of phospholipase C (U-73122). Also, the effect of bradykinin on cell proliferation in BCEC was evaluated using cell counts. RESULTS: In BCEC, [Ca2+]i in the resting state was 87 +/- 9 nM. Bradykinin induced an increment of [Ca2+]i in a concentration-dependent manner and its 50% effective concentration was approximately 5 x 10(-11) M. A [Ca2+]i increment at 10(-8) M bradykinin was inhibited with the pretreatment of EGTA, an extracellular calcium chelator. U-73122 (5 x 10(-6) M) attenuated the bradykinin-induced [Ca2+]i increment. The pretreatment of HOE-140 (Bk2 antagonist) almost attenuated the bradykinin (10(-8) M)-induced [Ca2+]i increase, but des-Arg9-[Leu(8)]-bradykinin (Bk1 antagonist) did not suppress it. To investigate the physiological effect of bradykinin, the effect of bradykinin on cell proliferation was studied. 10(-8) M of bradykinin produced a significant increase in cell numbers. This mitogenic effect of bradykinin was inhibited by the Bk2 antagonist. CONCLUSIONS: Bradykinin-induced stimulation of the signal transduction pathway in BCEC is coupled with the Bk2 type receptor. Furthermore, bradykinin produces the mitogenic effect in BCEC.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/142429
DOI
10.1159/000050879
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Medical Research Center
Yonsei Authors
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 http://www.karger.com/Article/FullText/50879
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