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Emergence of YMDD motif mutant of hepatitis B virus during short-term lamivudine therapy in South Korea

 Yong Han Paik  ;  Hyo Young Chung  ;  Wang-Shick Ryu  ;  Kwan Sik Lee  ;  Ji Sook Lee  ;  Jung Hee Kim  ;  Chun Kyun Lee  ;  Chae Yoon Chon  ;  Young Myoung Moon  ;  Kwang-Hyub Han 
 JOURNAL OF HEPATOLOGY, Vol.35(1) : 92-98, 2001 
Journal Title
Issue Date
Adult ; DNA, Viral/genetics ; Drug Administration Schedule ; Drug Resistance, Microbial/genetics* ; Female ; Hepatitis B/drug therapy* ; Hepatitis B virus/genetics* ; Humans ; Korea ; Lamivudine/administration & dosage ; Lamivudine/therapeutic use* ; Male ; Middle Aged ; Polymerase Chain Reaction/methods ; Prospective Studies ; Reverse Transcriptase Inhibitors/administration & dosage ; Reverse Transcriptase Inhibitors/therapeutic use* ; Sequence Analysis, DNA ; Time Factors
Chronic hepatitis B ; Lamivudine ; Hepatitis B virus DNA polymerase ; YMDD mutant
BACKGROUND/AIMS: The emergence of a YMDD mutant resistant to lamivudine therapy has been reported in patients with hepatitis B treated with long-term lamivudine therapy. However, it is not well known whether the YMDD mutant could be detected early in lamivudine therapy in hepatitis B virus (HBV) endemic areas. The aim of this study was to investigate the emergence of the YMDD mutant during short-term lamivudine therapy in South Korea. METHODS: We prospectively investigated the emergence of the YMDD mutant by the nested PCR assay using restriction fragment length polymorphism in 28 patients with chronic hepatitis B who were treated with 100 mg of lamivudine daily for 12 weeks. RESULTS: The YMDD mutant was detected in 17 (60.7%) out of 28 patients at week 12, and the only type of mutation found was the YIDD mutation. When we carried out the nested PCR serially in five patients, YIDD mutants were detected as early as 2 weeks by the nested PCR assay. The nested PCR results were in concordance with DNA sequencing in one patient's serial samples. CONCLUSIONS: YMDD mutants in HBV were detected within a few weeks during lamivudine therapy in South Korea, which suggests that the YMDD mutant may exist even before lamivudine therapy in HBV endemic areas.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Paik, Yong Han(백용한)
Lee, Kwan Sik(이관식) ORCID logo https://orcid.org/0000-0002-3672-1198
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