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추간판 퇴행에 따른 인간의 추간판 세포의 기질 합성 : 기저 상태 및 TGF-β1 자극에 따른 반응

Other Titles
 Matric synthesis of human intervertebral disc cells according to grade of degeneration: under the basal state and TGF-b1 stimulation 
 문성환  ;  김학선  ;  김남현  ;  박문수  ;  김향  ;  강응식  ;  박진오  ;  이환모  ;  전재훈 
 Journal of Korean Society of Spine Surgery (대한척추외과학회지), Vol.8(2) : 107-112, 2001 
Journal Title
 Journal of Korean Society of Spine Surgery (대한척추외과학회지) 
Issue Date
Disc degeneration ; TGF-β1 ; Proteoglycan
Study Design : In vitro experiment to determine the matrix synthesis of human intervertebral disc (IVD) cell according to grade of degeneration. Objectives : To quantify proteoglycan synthesis of human IVD cells in various grade of degeneration under the stimulation of TGF-β1. Summary of Literature Review : Sophisticated method to delivery of growth factors, in continuous manner, is the genetic modification of disc cells through gene transfer. Poor responsiveness of degenerated disc to anabolic stimuli can mitigate potential application of growth factor or therapeutic gene transfer in the management of degenerative disc disease Materials and Methods : IVD tissue was obtained from nineteen patients during surgical disc procedure. Grade of degeneration was confirmed by preoperative MRI. Isolation and three dimensional culture of disc cells were performed. Disc cells were treated with exogenous TGF-β1. Newly synthesized proteoglycans were assessed by 35S-sulfate incorporation using chromatography on Sepadex g-25 in PD-10 columns. One-way analysis of variance with Fishers protected LSD post-hoc test was performed to compare amount of newly synthesized proteoglycans and power analysis was also conducted. Significance level was set p<0.05. Results : Difference between cultures of control (grade Ⅰ) and degenerated (grade Ⅱ-Ⅴ) discs in proteoglycan synthesis in basal condition was statistically insignificant (p=0.35, power=0.21-0.63). Difference between cultures of control and degenerated disc in proteoglycan synthesis under the stimulated condition with TGF-β1 was also statistically insignificant (p=0.54, power=0.24-0.47). However cultures in stimulated condition showed increased amount of newly synthesized proteoglycans compared to those of basal condition regardless of the grade of degeneration(p<0.05). Conclusion : Anabolic response of human intervertebral disc cells is relatively insensitive to grade of disc degeneration, which facilitate application of gene therapy in various conditions of disc degeneration.
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1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
Moon, Seong Hwan(문성환)
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