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사람 코점막 배세포에서 MUC5AC mRNA의 발현 양상

Other Titles
 Expression of MUC5AC mRNA in the Goblet Cells of Human Nasal Mucosa 
 강성석  ;  김창훈  ;  김현웅  ;  이정권  ;  윤주헌 
 Korean Journal of Otolaryngology (대한이비인후과학회지), Vol.44(4) : 388-391, 2001 
Journal Title
 Korean Journal of Otolaryngology (대한이비인후과학회지) 
Issue Date
MUC5AC mRNA ; Goblet cell ; Human nasal mucosa
Background and Objectives:Mucus hypersecretion is a common feature in chronic sinusitis with polyps. Since mucus hypersecretion is commonly accompanied by goblet cell hyperplasia, it is important to identify which mucin gene mRNAs are expressed in the goblet cells of the surface epithelium in the human airway. This study aims to investigate the pattern of expression of MUC5AC mRNA in the goblet cells of human nasal mucosa. Materials and Methods:Six nasal polyps, five inferior turbinate mucosa specimens, and three normal-appearing mucosa specimens of the posterior ethmoid sinus were obtained. Each of the specimens were cut into 10 μm-thick serial frozen sections and in situ hybridization of MUC5AC mRNA was performed with an oligonucleotide probe. Alcian blue (pH 2.5)-periodic acid-Schiff staining was performed on the serial sections. Results:In human nasal polyps, MUC5AC mRNA was expressed in the cytoplasm of most of the goblet cells. However, in the inferior turbinate, MUC5AC mRNA was expressed in only some of the goblet cells. On the contrary, in the normal appearing mucosa of the posterior ethmoid sinus, MUC5AC mRNA was barely expressed in the goblet cells. Furthermore, MUC5AC mRNA was mainly expressed in some of the PAS-positive goblet cells. Conclusion:Only a portion of the goblet cells in the human nasal mucosa expressed MUC5AC mRNA. This result suggests that surface goblet cells might have other mucin genes in addition to MUC2 and MUC5AC.
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1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chang Hoon(김창훈) ORCID logo https://orcid.org/0000-0003-1238-6396
Yoon, Joo Heon(윤주헌)
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