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배양된 사람 정상 코점막 상피세포에서 Interleukin-13이 MUC5AC 유전자 발현 및 점액분비에 미치는 영향

Other Titles
 Interleukin-13 Suppresses MUC5AC Gene Expression and Mucin Secretion in Cultured Normal Human Nasal Epithelial Cells 
Authors
 이정권 ; 김창훈 ; 윤주헌 ; 배정호 ; 최재영 ; 김현웅 
Citation
 Korean Journal of Otorhinolaryngology-Head and Neck Surgery (대한이비인후과학회지 두경부외과학), Vol.44(11) : 1150~1156, 2001 
Journal Title
 Korean Journal of Otorhinolaryngology-Head and Neck Surgery (대한이비인후과학회지 두경부외과학) 
ISSN
 1225-035X 
Issue Date
2001
Abstract
Background and Objectives:It is not clear which mucins are responsible for the mucus hypersecretion in allergic rhinitis and allergic asthma. Recently, it was found that IL-13 is closely related to allergic inflammation and mucus hypersecretion in in vivo animal models. However, the role of IL-13 in in vitro cellular models has yet to be determined. This study aimed to investigate the direct effect of IL-13 on mucin gene expression and mucin secretion in cultured normal human nasal epithelial cells. Materials and Method:Cells were cultured with passage-2 normal human nasal epithelial cells using the air-liquid interface culture method. After treatment with IL-13, the total mucin and MUC5AC mucin levels were measured using the immuno-blotting assay. The MUC2, MUC5AC, and MUC8 mRNA expressions were measured using RT-PCR. Immunostaining was also performed using a MUC5AC antibody on histologic and cytospin slides. Results:After treatment with 5 ng/ml or more of IL-13, the level of total mucin and MUC5AC mucin secretion decreased substantially. The expression of MUC2 and MUC8 mRNA increased with higher concentrations of IL-13, but the expression of MUC5AC mRNA decreased. On the 7th day after IL-13 treatment, a significant decrease in the number of MUC5AC-positive cells was confirmed with immunostaining. Conclusion:These findings indicate that IL-13 suppresses MUC5AC mucin gene expression and mucin secretion in cultured normal human nasal epithelial cells in vitro.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/142236
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Otorhinolaryngology
Yonsei Authors
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