618 369

Cited 0 times in

배양된 사람 정상 코점막 상피세포에서 Interleukin-13이 MUC5AC 유전자 발현 및 점액분비에 미치는 영향

Other Titles
 Interleukin-13 Suppresses MUC5AC Gene Expression and Mucin Secretion in Cultured Normal Human Nasal Epithelial Cells 
 이정권  ;  김창훈  ;  김현웅  ;  최재영  ;  배정호  ;  윤주헌 
 Korean Journal of Otolaryngology (대한이비인후과학회지), Vol.44(11) : 1150-1156, 2001 
Journal Title
Korean Journal of Otolaryngology(대한이비인후과학회지)
Issue Date
Interleukin-13 ; Mucins ; MUC5AC
Background and Objectives:It is not clear which mucins are responsible for the mucus hypersecretion in allergic rhinitis and allergic asthma. Recently, it was found that IL-13 is closely related to allergic inflammation and mucus hypersecretion in in vivo animal models. However, the role of IL-13 in in vitro cellular models has yet to be determined. This study aimed to investigate the direct effect of IL-13 on mucin gene expression and mucin secretion in cultured normal human nasal epithelial cells.

Materials and Method:Cells were cultured with passage-2 normal human nasal epithelial cells using the air-liquid interface culture method. After treatment with IL-13, the total mucin and MUC5AC mucin levels were measured using the immuno-blotting assay. The MUC2, MUC5AC, and MUC8 mRNA expressions were measured using RT-PCR. Immunostaining was also performed using a MUC5AC antibody on histologic and cytospin slides.

Results:After treatment with 5 ng/ml or more of IL-13, the level of total mucin and MUC5AC mucin secretion decreased substantially. The expression of MUC2 and MUC8 mRNA increased with higher concentrations of IL-13, but the expression of MUC5AC mRNA decreased. On the 7th day after IL-13 treatment, a significant decrease in the number of MUC5AC-positive cells was confirmed with immunostaining.

Conclusion:These findings indicate that IL-13 suppresses MUC5AC mucin gene expression and mucin secretion in cultured normal human nasal epithelial cells in vitro.
Files in This Item:
T200101860.pdf Download
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chang Hoon(김창훈) ORCID logo https://orcid.org/0000-0003-1238-6396
Yoon, Joo Heon(윤주헌)
Lee, Jeung Gweon(이정권)
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.