cell culture ; cytotoxicity ; polyvinyl siloxane impression material ; agar overaly test
Abstract
The purpose of this study was to evaluate biocompatibility of polyvinyl siloxane impression materials and it's components using cell culture techniques. In the cytotoxicity test, using the culture of L929 mouse fibroblast, the degree of gross
cytotoxic
effects on the cells by changes in the cell morphology was scored for each materials. And the changes in the cell membrane permeability was tested using the agar overlay test. The results were as follows:
1. The effect of Examix impression material had on cell viability by evaluation survival rates was more severe than any other materials and newly added surfactants.
2. NP-4, nonylphenoxy polyethylene ethanol homologs, showed some cytotoxicy than PC-550 and BYK066.
3. In the test of the change in cell membrane permeability using the agar overlay method, any cytotoxicity was recorded in silicone base groups.
4. The application of surfactant such as PC-550, NP-4, and BYK066 might be considered relatively biocompatible.