16 45

Cited 0 times in

TGF-β1이 사람의 말초혈액 단구에서 랑게르한스세포로의 분화에 미치는 영향

Other Titles
 The Effect of TGF-β1 on the Differentiation of Langerhans Cells from Human Peripheral Blood Mononuclear Cells 
 신종란 ; 강진문 ; 이민걸 
 Korean Journal of InvestigativeDermatology (대한피부연구학회지), Vol.8(3) : 163~168, 2001 
Journal Title
 Korean Journal of InvestigativeDermatology (대한피부연구학회지) 
Issue Date
Langerhans cells (LC), dendritic cells (DC) in the epidermis, are potent antigen presenting cells. Epidermal LC can be generated in vitro from CD34+ hematopoietic cell, dermal DC can be generated in vitro from CD14+ mononuclear cells. Since CD14+ mononuclear cells constitutes about 5 to 10% of the peripheral blood mononuclear cells, and CD34+ cells less than 0.1%, it would greatly facilitate the study of Langerhans cells if they could be generated from the CD14+ mononuclear cells. It is well known that TGF-β1 is a critical cytokine for differentiation of LC. Recently, it has been demonstrated that TGF-β1 can induce further further differentiation of monocute derived dendritic cells to epidermal LC although some do not agree that TGF-β1-treated monocyte-derived DC are identical to LC. Our intention was to culture LC from human peripheral blood monocytes using TGF-β1 and compare their characteristics with those of the monocyte-derived DC. Human peripheral blood monocytes were cultured in the presence of ① GM-CSF, IL-4, ② GM-CSF, IL-4 and TGF-β1 (10 ng/㎖), ③ GM-CSF, IL-4 and TGF-β1 (10 ng/㎖) for 7 days. CD1a, CLA, E-cadherin expressions on DC were increased in the presence of TGF-β1 and their expressions were proportionally increased according to the increasing concentrations of TGF-β1. However, the expressions of CD83 and HLA-DR on DC were decreased when TGF-β1 was added. Endocytic capacity was assessed by examining DCs incubated with FITC-dextran using flow cytometry and the capacity was slightly decreased when TGF-β1 was added to the culture. Our data suggest that LC may be induced from peripheral blood monocyte in response to TGF-β1 stimulation.
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Dermatology
Yonsei Authors
사서에게 알리기
Files in This Item:
RIS (EndNote)
XLS (Excel)


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.