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TGF-β1이 사람의 말초혈액 단구에서 랑게르한스세포로의 분화에 미치는 영향

Other Titles
 The Effect of TGF-β1 on the Differentiation of Langerhans Cells from Human Peripheral Blood Mononuclear Cells 
Authors
 신종란 ; 강진문 ; 이민걸 
Citation
 Korean Journal of InvestigativeDermatology (대한피부연구학회지), Vol.8(3) : 163~168, 2001 
Journal Title
 Korean Journal of InvestigativeDermatology (대한피부연구학회지) 
ISSN
 1225-8180 
Issue Date
2001
Abstract
Langerhans cells (LC), dendritic cells (DC) in the epidermis, are potent antigen presenting cells. Epidermal LC can be generated in vitro from CD34+ hematopoietic cell, dermal DC can be generated in vitro from CD14+ mononuclear cells. Since CD14+ mononuclear cells constitutes about 5 to 10% of the peripheral blood mononuclear cells, and CD34+ cells less than 0.1%, it would greatly facilitate the study of Langerhans cells if they could be generated from the CD14+ mononuclear cells. It is well known that TGF-β1 is a critical cytokine for differentiation of LC. Recently, it has been demonstrated that TGF-β1 can induce further further differentiation of monocute derived dendritic cells to epidermal LC although some do not agree that TGF-β1-treated monocyte-derived DC are identical to LC. Our intention was to culture LC from human peripheral blood monocytes using TGF-β1 and compare their characteristics with those of the monocyte-derived DC. Human peripheral blood monocytes were cultured in the presence of ① GM-CSF, IL-4, ② GM-CSF, IL-4 and TGF-β1 (10 ng/㎖), ③ GM-CSF, IL-4 and TGF-β1 (10 ng/㎖) for 7 days. CD1a, CLA, E-cadherin expressions on DC were increased in the presence of TGF-β1 and their expressions were proportionally increased according to the increasing concentrations of TGF-β1. However, the expressions of CD83 and HLA-DR on DC were decreased when TGF-β1 was added. Endocytic capacity was assessed by examining DCs incubated with FITC-dextran using flow cytometry and the capacity was slightly decreased when TGF-β1 was added to the culture. Our data suggest that LC may be induced from peripheral blood monocyte in response to TGF-β1 stimulation.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/142110
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Dermatology
Yonsei Authors
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