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TGF-β1이 사람의 말초혈액 단구에서 랑게르한스세포로의 분화에 미치는 영향

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dc.contributor.author이민걸-
dc.date.accessioned2016-02-19T10:58:25Z-
dc.date.available2016-02-19T10:58:25Z-
dc.date.issued2001-
dc.identifier.issn1225-8180-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/142110-
dc.description.abstractLangerhans cells (LC), dendritic cells (DC) in the epidermis, are potent antigen presenting cells. Epidermal LC can be generated in vitro from CD34+ hematopoietic cell, dermal DC can be generated in vitro from CD14+ mononuclear cells. Since CD14+ mononuclear cells constitutes about 5 to 10% of the peripheral blood mononuclear cells, and CD34+ cells less than 0.1%, it would greatly facilitate the study of Langerhans cells if they could be generated from the CD14+ mononuclear cells. It is well known that TGF-β1 is a critical cytokine for differentiation of LC. Recently, it has been demonstrated that TGF-β1 can induce further further differentiation of monocute derived dendritic cells to epidermal LC although some do not agree that TGF-β1-treated monocyte-derived DC are identical to LC. Our intention was to culture LC from human peripheral blood monocytes using TGF-β1 and compare their characteristics with those of the monocyte-derived DC. Human peripheral blood monocytes were cultured in the presence of ① GM-CSF, IL-4, ② GM-CSF, IL-4 and TGF-β1 (10 ng/㎖), ③ GM-CSF, IL-4 and TGF-β1 (10 ng/㎖) for 7 days. CD1a, CLA, E-cadherin expressions on DC were increased in the presence of TGF-β1 and their expressions were proportionally increased according to the increasing concentrations of TGF-β1. However, the expressions of CD83 and HLA-DR on DC were decreased when TGF-β1 was added. Endocytic capacity was assessed by examining DCs incubated with FITC-dextran using flow cytometry and the capacity was slightly decreased when TGF-β1 was added to the culture. Our data suggest that LC may be induced from peripheral blood monocyte in response to TGF-β1 stimulation.-
dc.description.statementOfResponsibilityopen-
dc.format.extent163~168-
dc.relation.isPartOfKorean Journal of InvestigativeDermatology (대한피부연구학회지)-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleTGF-β1이 사람의 말초혈액 단구에서 랑게르한스세포로의 분화에 미치는 영향-
dc.title.alternativeThe Effect of TGF-β1 on the Differentiation of Langerhans Cells from Human Peripheral Blood Mononuclear Cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Dermatology (피부과학)-
dc.contributor.googleauthor신종란-
dc.contributor.googleauthor강진문-
dc.contributor.googleauthor이민걸-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02779-
dc.relation.journalcodeJ02042-
dc.subject.keywordlangerhans cells-
dc.subject.keyworddendritic cells-
dc.subject.keywordTGF-β1-
dc.contributor.alternativeNameLee, Min Geol-
dc.contributor.affiliatedAuthorLee, Min Geol-
dc.rights.accessRightsfree-
dc.citation.volume8-
dc.citation.number3-
dc.citation.startPage163-
dc.citation.endPage168-
dc.identifier.bibliographicCitationKorean Journal of InvestigativeDermatology (대한피부연구학회지), Vol.8(3) : 163-168, 2001-
dc.identifier.rimsid31611-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers

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