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TGF-β1이 사람의 말초혈액 단구에서 랑게르한스세포로의 분화에 미치는 영향
DC Field | Value | Language |
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dc.contributor.author | 이민걸 | - |
dc.date.accessioned | 2016-02-19T10:58:25Z | - |
dc.date.available | 2016-02-19T10:58:25Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 1225-8180 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/142110 | - |
dc.description.abstract | Langerhans cells (LC), dendritic cells (DC) in the epidermis, are potent antigen presenting cells. Epidermal LC can be generated in vitro from CD34+ hematopoietic cell, dermal DC can be generated in vitro from CD14+ mononuclear cells. Since CD14+ mononuclear cells constitutes about 5 to 10% of the peripheral blood mononuclear cells, and CD34+ cells less than 0.1%, it would greatly facilitate the study of Langerhans cells if they could be generated from the CD14+ mononuclear cells. It is well known that TGF-β1 is a critical cytokine for differentiation of LC. Recently, it has been demonstrated that TGF-β1 can induce further further differentiation of monocute derived dendritic cells to epidermal LC although some do not agree that TGF-β1-treated monocyte-derived DC are identical to LC. Our intention was to culture LC from human peripheral blood monocytes using TGF-β1 and compare their characteristics with those of the monocyte-derived DC. Human peripheral blood monocytes were cultured in the presence of ① GM-CSF, IL-4, ② GM-CSF, IL-4 and TGF-β1 (10 ng/㎖), ③ GM-CSF, IL-4 and TGF-β1 (10 ng/㎖) for 7 days. CD1a, CLA, E-cadherin expressions on DC were increased in the presence of TGF-β1 and their expressions were proportionally increased according to the increasing concentrations of TGF-β1. However, the expressions of CD83 and HLA-DR on DC were decreased when TGF-β1 was added. Endocytic capacity was assessed by examining DCs incubated with FITC-dextran using flow cytometry and the capacity was slightly decreased when TGF-β1 was added to the culture. Our data suggest that LC may be induced from peripheral blood monocyte in response to TGF-β1 stimulation. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 163~168 | - |
dc.relation.isPartOf | Korean Journal of InvestigativeDermatology (대한피부연구학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | TGF-β1이 사람의 말초혈액 단구에서 랑게르한스세포로의 분화에 미치는 영향 | - |
dc.title.alternative | The Effect of TGF-β1 on the Differentiation of Langerhans Cells from Human Peripheral Blood Mononuclear Cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Dermatology (피부과학) | - |
dc.contributor.googleauthor | 신종란 | - |
dc.contributor.googleauthor | 강진문 | - |
dc.contributor.googleauthor | 이민걸 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02779 | - |
dc.relation.journalcode | J02042 | - |
dc.subject.keyword | langerhans cells | - |
dc.subject.keyword | dendritic cells | - |
dc.subject.keyword | TGF-β1 | - |
dc.contributor.alternativeName | Lee, Min Geol | - |
dc.contributor.affiliatedAuthor | Lee, Min Geol | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 8 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 163 | - |
dc.citation.endPage | 168 | - |
dc.identifier.bibliographicCitation | Korean Journal of InvestigativeDermatology (대한피부연구학회지), Vol.8(3) : 163-168, 2001 | - |
dc.identifier.rimsid | 31611 | - |
dc.type.rims | ART | - |
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