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HNF1 and/or HNF3 may contribute to the tissue specific expression of glucokinase gene

Authors
 Ji-Young Cha  ;  Ha-il Kim  ;  Seung-Soon Im  ;  Tian-Zhu Li  ;  Yong-ho Ahn 
Citation
 EXPERIMENTAL AND MOLECULAR MEDICINE, Vol.33(2) : 59-63, 2001 
Journal Title
 EXPERIMENTAL AND MOLECULAR MEDICINE 
ISSN
 1226-3613 
Issue Date
2001
MeSH
3T3 Cells ; Animals ; Blotting, Northern ; Cell Line ; Cell Nucleus/metabolism ; Cells, Cultured ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/physiology* ; Genes, Reporter ; Glucokinase/biosynthesis* ; Glucokinase/genetics* ; HeLa Cells ; Hepatocyte Nuclear Factor 1 ; Hepatocyte Nuclear Factor 1-alpha ; Hepatocyte Nuclear Factor 1-beta ; Hepatocyte Nuclear Factor 3-beta ; Humans ; Liver/metabolism ; Luciferases/metabolism ; Mice ; Models, Genetic ; Nuclear Proteins/genetics ; Nuclear Proteins/physiology* ; Plasmids/metabolism ; Promoter Regions, Genetic ; Protein Binding ; Rats ; Tissue Distribution ; Transcription Factors/genetics ; Transcription Factors/physiology* ; Transcription, Genetic ; Transfection
Keywords
GK ; GLUT2 ; HNF1 ; HNF3
Abstract
A possible role of hepatocyte nuclear factor 1 (HNF1) or HNF3, a predominant trans-acting factors of hepatic or pancreatic β-cells, was examined on the tissue specific interdependent expression of glucokinase (GK) in liver, H4IIE, HepG2, HIT-T15 and MIN6 cell line. The tissues or cell lines known to express GK showed abundant levels of HNF1 and HNF3 mRNA as observed in liver, H4IIE, HepG2, HIT-T15 and MIN6 cells, whereas they were not detected in brain, heart, NIH 3T3, HeLa cells. The promoter of glucokinase contains several HNF3 consensus sequences and are well conserved in human, mouse and rat. Transfection of the glucokinase promotor linked with luciferase reporter to liver or pancreatic β cell lines showed high interacting activities with HNF1 and HNF3, whereas minimal activities were detected in the cells expressing very low levels of HNFs. The binding of HNF1 or HNF3 to the GK promoter genes was confirmed by electrophoretic mobility shift assay (EMSA). From these data, we propose that the expression of HNF1 and/or HNF3 may, in part, contribute to the tissue specific expression of GK.
Files in This Item:
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DOI
10.1038/emm.2001.11
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Ahn, Yong Ho(안용호) ORCID logo https://orcid.org/0000-0002-4133-0757
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/142106
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