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HNF1 and/or HNF3 may contribute to the tissue specific expression of glucokinase gene

DC FieldValueLanguage
dc.contributor.author안용호-
dc.date.accessioned2016-02-19T10:58:18Z-
dc.date.available2016-02-19T10:58:18Z-
dc.date.issued2001-
dc.identifier.issn1226-3613-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/142106-
dc.description.abstractA possible role of hepatocyte nuclear factor 1 (HNF1) or HNF3, a predominant trans-acting factors of hepatic or pancreatic β-cells, was examined on the tissue specific interdependent expression of glucokinase (GK) in liver, H4IIE, HepG2, HIT-T15 and MIN6 cell line. The tissues or cell lines known to express GK showed abundant levels of HNF1 and HNF3 mRNA as observed in liver, H4IIE, HepG2, HIT-T15 and MIN6 cells, whereas they were not detected in brain, heart, NIH 3T3, HeLa cells. The promoter of glucokinase contains several HNF3 consensus sequences and are well conserved in human, mouse and rat. Transfection of the glucokinase promotor linked with luciferase reporter to liver or pancreatic β cell lines showed high interacting activities with HNF1 and HNF3, whereas minimal activities were detected in the cells expressing very low levels of HNFs. The binding of HNF1 or HNF3 to the GK promoter genes was confirmed by electrophoretic mobility shift assay (EMSA). From these data, we propose that the expression of HNF1 and/or HNF3 may, in part, contribute to the tissue specific expression of GK.-
dc.description.statementOfResponsibilityopen-
dc.format.extent59~63-
dc.relation.isPartOfEXPERIMENTAL AND MOLECULAR MEDICINE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESH3T3 Cells-
dc.subject.MESHAnimals-
dc.subject.MESHBlotting, Northern-
dc.subject.MESHCell Line-
dc.subject.MESHCell Nucleus/metabolism-
dc.subject.MESHCells, Cultured-
dc.subject.MESHDNA-Binding Proteins/genetics-
dc.subject.MESHDNA-Binding Proteins/physiology*-
dc.subject.MESHGenes, Reporter-
dc.subject.MESHGlucokinase/biosynthesis*-
dc.subject.MESHGlucokinase/genetics*-
dc.subject.MESHHeLa Cells-
dc.subject.MESHHepatocyte Nuclear Factor 1-
dc.subject.MESHHepatocyte Nuclear Factor 1-alpha-
dc.subject.MESHHepatocyte Nuclear Factor 1-beta-
dc.subject.MESHHepatocyte Nuclear Factor 3-beta-
dc.subject.MESHHumans-
dc.subject.MESHLiver/metabolism-
dc.subject.MESHLuciferases/metabolism-
dc.subject.MESHMice-
dc.subject.MESHModels, Genetic-
dc.subject.MESHNuclear Proteins/genetics-
dc.subject.MESHNuclear Proteins/physiology*-
dc.subject.MESHPlasmids/metabolism-
dc.subject.MESHPromoter Regions, Genetic-
dc.subject.MESHProtein Binding-
dc.subject.MESHRats-
dc.subject.MESHTissue Distribution-
dc.subject.MESHTranscription Factors/genetics-
dc.subject.MESHTranscription Factors/physiology*-
dc.subject.MESHTranscription, Genetic-
dc.subject.MESHTransfection-
dc.titleHNF1 and/or HNF3 may contribute to the tissue specific expression of glucokinase gene-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Biochemistry & Molecular Biology (생화학,분자생물학)-
dc.contributor.googleauthorJi-Young Cha-
dc.contributor.googleauthorHa-il Kim-
dc.contributor.googleauthorSeung-Soon Im-
dc.contributor.googleauthorTian-Zhu Li-
dc.contributor.googleauthorYong-ho Ahn-
dc.identifier.doi10.1038/emm.2001.11-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02249-
dc.relation.journalcodeJ00860-
dc.identifier.eissn2092-6413-
dc.identifier.pmid11460882-
dc.subject.keywordGK-
dc.subject.keywordGLUT2-
dc.subject.keywordHNF1-
dc.subject.keywordHNF3-
dc.contributor.alternativeNameAhn, Yong Ho-
dc.contributor.affiliatedAuthorAhn, Yong Ho-
dc.rights.accessRightsfree-
dc.citation.volume33-
dc.citation.number2-
dc.citation.startPage59-
dc.citation.endPage63-
dc.identifier.bibliographicCitationEXPERIMENTAL AND MOLECULAR MEDICINE, Vol.33(2) : 59-63, 2001-
dc.identifier.rimsid31607-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers

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