251 292

Cited 0 times in

Oligoclonal B lymphocyte expansion in the synovium of a patient with Behçet's disease

 Chang Hee Suh  ;  Yong Beom Park  ;  Jungsik Song  ;  Chan Hee Lee  ;  Soo Kon Lee 
 Arthritis & Rheumatism, Vol.44(7) : 1707-1712, 2001 
Journal Title
Arthritis & Rheumatism
Issue Date
Adult ; B-Lymphocytes/immunology* ; Base Sequence ; Behcet Syndrome/immunology* ; Complementarity Determining Regions/genetics* ; Complementarity Determining Regions/immunology ; Female ; Humans ; Immunoglobulins/genetics ; Immunoglobulins/immunology ; Molecular Sequence Data ; Mutation/immunology ; Oligoclonal Bands ; Synovial Membrane/immunology*
Plasma cell infiltration is observed in recurrent arthritis associated with Behçet's disease (BD). The immune mechanism underlying B lymphocyte proliferation in the synovium is unclear. One hypothesis involves nonspecific polyclonal activation and another involves antigen-driven activation. The present study was undertaken to test both hypotheses and identify immunoglobulin genes that are clonally expanded in the synovium.
Peripheral blood lymphocytes (PBL) and synovial cells from a patient with BD and PBL from a healthy control subject were obtained. Complementarity-determining region 3 (CDR3) fingerprinting analysis and nucleotide sequence analysis of Ig transcripts derived from clonally expanded B lymphocytes were performed in parallel.
Of 44 mu heavy chain clones of the VH4 family identified in the synovial tissue from the BD patient, 8 clones showed identical nucleotide sequences, and therefore, 18.2% were clonally expanded. For y heavy chain, 4 of 50 clones of the VH3 family showed nearly identical sequences; therefore, 4-8% were clonally expanded. The kappa light chain did not show a dominant band, but a clone with a 12-amino acid CDR3 showed 3% clonal expansion. Somatic mutations were frequently observed, with a high ratio of replacement to silent mutations in the CDRs compared with the framework regions. Three Ig genes expressed in the clonally expanded B lymphocytes were derived from germline gene segments reported to be involved in the production of autoantibodies.
These results support the hypothesis that antigen-driven clonal B lymphocyte proliferation occurs in the synovium in BD. Immunoglobulin transcripts clonally expanded in the synovium were identified.
Files in This Item:
T200101476.pdf Download
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Park, Yong Beom(박용범)
Lee, Soo Kon(이수곤)
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.