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Oligoclonal B lymphocyte expansion in the synovium of a patient with Behçet's disease
DC Field | Value | Language |
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dc.contributor.author | 박용범 | - |
dc.contributor.author | 이수곤 | - |
dc.date.accessioned | 2016-02-19T10:56:31Z | - |
dc.date.available | 2016-02-19T10:56:31Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 0004-3591 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/142041 | - |
dc.description.abstract | OBJECTIVE: Plasma cell infiltration is observed in recurrent arthritis associated with Behçet's disease (BD). The immune mechanism underlying B lymphocyte proliferation in the synovium is unclear. One hypothesis involves nonspecific polyclonal activation and another involves antigen-driven activation. The present study was undertaken to test both hypotheses and identify immunoglobulin genes that are clonally expanded in the synovium. METHODS: Peripheral blood lymphocytes (PBL) and synovial cells from a patient with BD and PBL from a healthy control subject were obtained. Complementarity-determining region 3 (CDR3) fingerprinting analysis and nucleotide sequence analysis of Ig transcripts derived from clonally expanded B lymphocytes were performed in parallel. RESULTS: Of 44 mu heavy chain clones of the VH4 family identified in the synovial tissue from the BD patient, 8 clones showed identical nucleotide sequences, and therefore, 18.2% were clonally expanded. For y heavy chain, 4 of 50 clones of the VH3 family showed nearly identical sequences; therefore, 4-8% were clonally expanded. The kappa light chain did not show a dominant band, but a clone with a 12-amino acid CDR3 showed 3% clonal expansion. Somatic mutations were frequently observed, with a high ratio of replacement to silent mutations in the CDRs compared with the framework regions. Three Ig genes expressed in the clonally expanded B lymphocytes were derived from germline gene segments reported to be involved in the production of autoantibodies. CONCLUSION: These results support the hypothesis that antigen-driven clonal B lymphocyte proliferation occurs in the synovium in BD. Immunoglobulin transcripts clonally expanded in the synovium were identified. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1707~1712 | - |
dc.relation.isPartOf | Arthritis & Rheumatism | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adult | - |
dc.subject.MESH | B-Lymphocytes/immunology* | - |
dc.subject.MESH | Base Sequence | - |
dc.subject.MESH | Behcet Syndrome/immunology* | - |
dc.subject.MESH | Complementarity Determining Regions/genetics* | - |
dc.subject.MESH | Complementarity Determining Regions/immunology | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunoglobulins/genetics | - |
dc.subject.MESH | Immunoglobulins/immunology | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Mutation/immunology | - |
dc.subject.MESH | Oligoclonal Bands | - |
dc.subject.MESH | Synovial Membrane/immunology* | - |
dc.title | Oligoclonal B lymphocyte expansion in the synovium of a patient with Behçet's disease | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Chang Hee Suh | - |
dc.contributor.googleauthor | Yong Beom Park | - |
dc.contributor.googleauthor | Jungsik Song | - |
dc.contributor.googleauthor | Chan Hee Lee | - |
dc.contributor.googleauthor | Soo Kon Lee | - |
dc.identifier.doi | 10.1002/1529-0131(200107)44:7<1707::AID-ART295>3.0.CO;2-P | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01579 | - |
dc.contributor.localId | A02889 | - |
dc.relation.journalcode | J00239 | - |
dc.identifier.pmid | 11465723 | - |
dc.contributor.alternativeName | Park, Yong Beom | - |
dc.contributor.alternativeName | Lee, Soo Kon | - |
dc.contributor.affiliatedAuthor | Park, Yong Beom | - |
dc.contributor.affiliatedAuthor | Lee, Soo Kon | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 44 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 1707 | - |
dc.citation.endPage | 1712 | - |
dc.identifier.bibliographicCitation | Arthritis & Rheumatism, Vol.44(7) : 1707-1712, 2001 | - |
dc.identifier.rimsid | 31554 | - |
dc.type.rims | ART | - |
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