733 426

Cited 0 times in

근육모세포 배양에서 Proline Analogue와 Cytosine Arabinoside의 섬유모세포 제거 효과

Other Titles
 Selective Removal of Fibroblast with Using Proline Analogue andCytosine Arabinoside in Myoblast Culture 
Authors
 박윤길  ;  김진  ;  신정식  ;  문재호 
Citation
 Journal of the Korean Academy of Rehabilitation Medicine (대한재활의학회지), Vol.25(3) : 431-437, 2001 
Journal Title
Journal of the Korean Academy of Rehabilitation Medicine(대한재활의학회지)
ISSN
 1225-584X 
Issue Date
2001
Keywords
Myoblast ; Fibroblast ; Proline analogue ; Cytosine arabinoside
Abstract
Objective: The phenomenon of fibroblast overgrowth is one of the major problems encountered during long-term culture such as myoblast culture. The first goal of the study is to determine the effects of proline analogue and cytosine arabinoside to reduce fibroblasts in myoblast culture. The second goal is to investigate whether the chemicals influence the growth and differentiation of myoblast.

Method: Muscle tissues were obtained from legs of healthy men, and then fibroblasts and myoblasts were isolated and cultured. Those mixed cells were divided into three groups; control group, proline analogue (cis-hydroxyproline) treated group and cytosine arabinoside (araC) treated group. We evaluated the effectiveness of cis-hydroxyproline and araC on selective removal of fibroblasts in culture. We have also determined if cis-hydroxyproline and araC could alter differentiation of myoblast in each group.

Results: The treatment with araC was effective to eliminate fibroblasts comparing to the control group (p<0.05) while there was no statistically significant difference between proline analogue and control group (p>0.05). Myoblasts of all three groups were differentiated into myotube.

Conclusion: Using araC, we could reduce a number of fibroblasts in myoblast culture where contamination and subsequent overgrowth with fibroblasts remained a problem.
Files in This Item:
T200101427.pdf Download
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Rehabilitation Medicine (재활의학교실) > 1. Journal Papers
Yonsei Authors
Moon, Jae Ho(문재호)
Park, Yoon Ghil(박윤길) ORCID logo https://orcid.org/0000-0001-9054-5300
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/142015
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links