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Multifunctional nanoparticles for gene delivery and spinal cord injury

 So-Jung Gwak  ;  Heebeom Koo  ;  Yoon Ha  ;  Kwangmeyung Kim  ;  Do Heum Yoon  ;  Hye Yeong Lee  ;  Ji Young Yhee  ;  Yeomin Yun 
 Journal of Biomedical Materials Research Part A, Vol.103(11) : 3474-3782, 2015 
Journal Title
 Journal of Biomedical Materials Research Part A 
Issue Date
Animals ; Anti-Inflammatory Agents/pharmacology ; Apoptosis/drug effects ; Chitosan/pharmacology ; Gene Transfer Techniques* ; Luciferases/metabolism ; Male ; Methylprednisolone/pharmacology ; Mice ; Nanoparticles/chemistry* ; Neural Stem Cells/cytology ; Neural Stem Cells/drug effects ; Neural Stem Cells/metabolism ; Proton Magnetic Resonance Spectroscopy ; Rats, Sprague-Dawley ; Spinal Cord Injuries/pathology* ; Transfection ; beta-Galactosidase/metabolism
chitosan ; gene delivery ; methylprednisolone ; spinal cord injury
Methylprednisolone (MP) is a glucocorticoid that is used as an anti-inflammatory agent to the treat spinal cord injury (SCI). A low molecular weight chitosan was used to synthesize chitosan-MP conjugate, which was used to evaluate the gene therapy, anti-inflammatory and anti-apoptotic effects of MP. The cytotoxicity of chitosan-MP nanoparticles and the transfection efficiency of plasmid DNA were evaluated by MTT and luciferase assays. A chitosan-MP/pDNA complexes was injected into injured spinal cord to evaluate the anti-inflammatory and anti-apoptotic effects of these complexes using terminal deoxynucleotide transferase dUTP nick end labeling (TUNEL) and ED1 staining, respectively. In addition, to evaluate the distribution of chitosan-MP/pDNA complexes, pβ-gal encapsulated chitosan-MP was injected into the injected site. Cell survival was similar in cells treated with chitosan-MP conjugate and untreated cells. Luciferase expression was higher in cells treated with the chitosan-MP/pDNA than cells treated with the chitosan/pDNA. The chitosan-MP/pDNA complexes also reduced apoptosis and inflammation at the injury site. These results suggest that chitosan-MP conjugation is an effective gene delivery system to treat SCI. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3474-3482, 2015.
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Gwak, So Jung(곽소정)
Yoon, Do Heum(윤도흠) ORCID logo https://orcid.org/0000-0003-1452-5724
Lee, Hye Yeong(이혜영)
Ha, Yoon(하윤)
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