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Multifunctional nanoparticles for gene delivery and spinal cord injury

Authors
 So-Jung Gwak  ;  Heebeom Koo  ;  Yoon Ha  ;  Kwangmeyung Kim  ;  Do Heum Yoon  ;  Hye Yeong Lee  ;  Ji Young Yhee  ;  Yeomin Yun 
Citation
 Journal of Biomedical Materials Research Part A, Vol.103(11) : 3474-3782, 2015 
Journal Title
 Journal of Biomedical Materials Research Part A 
ISSN
 1549-3296 
Issue Date
2015
Abstract
Methylprednisolone (MP) is a glucocorticoid that is used as an anti-inflammatory agent to the treat spinal cord injury (SCI). A low molecular weight chitosan was used to synthesize chitosan-MP conjugate, which was used to evaluate the gene therapy, anti-inflammatory and anti-apoptotic effects of MP. The cytotoxicity of chitosan-MP nanoparticles and the transfection efficiency of plasmid DNA were evaluated by MTT and luciferase assays. A chitosan-MP/pDNA complexes was injected into injured spinal cord to evaluate the anti-inflammatory and anti-apoptotic effects of these complexes using terminal deoxynucleotide transferase dUTP nick end labeling (TUNEL) and ED1 staining, respectively. In addition, to evaluate the distribution of chitosan-MP/pDNA complexes, pβ-gal encapsulated chitosan-MP was injected into the injected site. Cell survival was similar in cells treated with chitosan-MP conjugate and untreated cells. Luciferase expression was higher in cells treated with the chitosan-MP/pDNA than cells treated with the chitosan/pDNA. The chitosan-MP/pDNA complexes also reduced apoptosis and inflammation at the injury site. These results suggest that chitosan-MP conjugation is an effective gene delivery system to treat SCI. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3474-3482, 2015.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/141825
DOI
10.1002/jbm.a.35489
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원)
Yonsei Authors
곽소정(Gwak, So Jung) ; 윤도흠(Yoon, Do Heum) ; 이혜영(Lee, Hye Yeong) ; 하윤(Ha, Yoon)
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Full Text
http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.35489/abstract
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