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Activation of MAPK Is Required for ROS Generation and Exocytosis in HMC-1 Cells Induced by Trichomonas vaginalis-Derived Secretory Products

 Giimaa Narantsogt  ;  Arim Min  ;  Young Hee Nam  ;  Young Ah Lee  ;  Kyeong Ah Kim  ;  Gurbadam Agvaandaram  ;  Temuulen Dorjsuren  ;  Jamel El-Benna  ;  Myeong Heon Shin 
 Korean Journal of Parasitology, Vol.53(5) : 597-603, 2015 
Journal Title
 Korean Journal of Parasitology 
Issue Date
Trichomonas vaginalis is a flagellated protozoan parasite that causes vaginitis and cervicitis in women and asymptomatic urethritis and prostatitis in men. Mast cells have been reported to be predominant in vaginal smears and vaginal walls of patients infected with T. vaginalis. Mitogen-activated protein kinase (MAPK), activated by various stimuli, have been shown to regulate the transcriptional activity of various cytokine genes in mast cells. In this study, we investigated whether MAPK is involved in ROS generation and exocytotic degranulation in HMC-1 cells induced by T. vaginalis-derived secretory products (TvSP). We found that TvSP induces the activation of MAPK and NADPH oxidase in HMC-1 cells. Stimulation with TvSP induced phosphorylation of MAPK and p47(phox) in HMC-1 cells. Stimulation with TvSP also induced up-regulation of CD63, a marker for exocytosis, along the surfaces of human mast cells. Pretreatment with MAPK inhibitors strongly inhibited TvSP-induced ROS generation and exocytotic degranulation. Finally, our results suggest that TvSP induces intracellular ROS generation and exocytotic degranulation in HMC-1 via MAPK signaling.
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1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
남영희(Nam, Young Hee)
민아림(Min, A Rim) ORCID logo https://orcid.org/0000-0003-2938-9630
신명헌(Shin, Myeong Heon) ORCID logo https://orcid.org/0000-0001-8207-6110
이영아(Lee, Young Ah) ORCID logo https://orcid.org/0000-0002-0414-842X
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