0 202

Cited 8 times in

Electro-hyperthermia inhibits glioma tumorigenicity through the induction of E2F1-mediated apoptosis

 Jihye Cha  ;  Tae-Won Jeon  ;  Chang Geol Lee  ;  Sang Taek Oh  ;  Hee-Beom Yang  ;  Kyung-Ju Choi  ;  Daekwan Seo  ;  Ina Yun  ;  In Hye Baik  ;  Kyung Ran Park  ;  Young Nyun Park  ;  Yun-Han Lee 
 International Journal of Hyperthermia, Vol.31(7) : 784-792, 2015 
Journal Title
 International Journal of Hyperthermia 
Issue Date
PURPOSE: Modulated electro-hyperthermia (mEHT), also known as oncothermia, shows remarkable treatment efficacies for various types of tumours, including glioma. The aim of the present study was to investigate the molecular mechanism underlying phenotypic changes in oncothermic cancer cells. MATERIALS AND METHODS: U87-MG and A172 human glioma cells were exposed to mEHT (42 °C/60 min) three times with a 2-day interval and subsequently tested for growth inhibition using MTS, FACS and microscopic analysis. To obtain insights into the molecular changes in response to mEHT, global changes in gene expression were examined using RNA sequencing. For in vivo evaluation of mEHT, we used U87-MG glioma xenografts grown in nude mice. RESULTS: mEHT inhibited glioma cell growth through the strong induction of apoptosis. The transcriptomic analysis of differential gene expression under mEHT showed that the anti-proliferative effects were induced through a subset of molecular alterations, including the up-regulation of E2F1 and CPSF2 and the down-regulation of ADAR and PSAT1. Subsequent Western blotting revealed that mEHT increased the levels of E2F1 and p53 and decreased the level of PARP-1, accelerating apoptotic signalling in glioma cells. mEHT significantly suppressed the growth of human glioma xenografts in nude mice. We also observed that mEHT dramatically reduced the portion of CD133(+) glioma stem cell population and suppressed cancer cell migration and sphere formation. CONCLUSIONS: These findings suggest that mEHT suppresses glioma cell proliferation and mobility through the induction of E2F1-mediated apoptosis and might be an effective treatment for eradicating brain tumours.
Full Text
Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers
Yonsei Authors
박영년(Park, Young Nyun) ORCID logo https://orcid.org/0000-0003-0357-7967
백인혜(Baik, In Hye)
이윤한(Lee, Yun Han)
이창걸(Lee, Chang Geol) ORCID logo https://orcid.org/0000-0002-8702-881X
차지혜(Cha, Ji Hye)
최경주(Choi, Kyung Ju)
RIS (EndNote)
XLS (Excel)
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.