114 276

Cited 14 times in

Phenotypic and genotypic analysis of anti-tuberculosis drug resistance in Mycobacterium tuberculosis isolates in Myanmar

 Wah Wah Aung  ;  Phyu Win Ei  ;  Wint Wint Nyunt  ;  Thyn Lei Swe  ;  Thandar Lwin  ;  Mi Mi Htwe  ;  Kyung Jun Kim  ;  Jong Seok Lee  ;  Chang Ki Kim  ;  Sang Nae Cho  ;  Sun Dae Song  ;  Chulhun L. Chang 
 ANNALS OF LABORATORY MEDICINE, Vol.35(5) : 494-499, 2015 
Journal Title
Issue Date
Adult ; Antitubercular Agents/pharmacology* ; DNA, Bacterial/genetics ; DNA, Bacterial/metabolism ; Drug Resistance, Multiple, Bacterial/genetics ; Female ; Genotype ; Humans ; Male ; Microbial Sensitivity Tests ; Middle Aged ; Myanmar ; Mycobacterium tuberculosis/drug effects* ; Mycobacterium tuberculosis/genetics ; Mycobacterium tuberculosis/isolation & purification ; Phenotype ; Polymerase Chain Reaction ; Sputum/microbiology ; Tuberculosis/diagnosis ; Tuberculosis/microbiology
Drug ; Genotype ; Myanmar ; Mycobacterium tuberculosis ; Resistance
BACKGROUND: Tuberculosis (TB) is one of the most serious health problems in Myanmar. Because TB drug resistance is associated with genetic mutation(s) relevant to responses to each drug, genotypic methods for detecting these mutations have been proposed to overcome the limitations of classic phenotypic drug susceptibility testing (DST). We explored the current estimates of drug-resistant TB and evaluated the usefulness of genotypic DST in Myanmar. METHODS: We determined the drug susceptibility of Mycobacterium tuberculosis isolated from sputum smear-positive patients with newly diagnosed pulmonary TB at two main TB centers in Myanmar during 2013 by using conventional phenotypic DST and the GenoType MTBDRplus assay (Hain Lifescience, Germany). Discrepant results were confirmed by sequencing the genes relevant to each type of resistance (rpoB for rifampicin; katG and inhA for isoniazid). RESULTS: Of 191 isolates, phenotypic DST showed that 27.7% (n=53) were resistant to at least one first-line drug and 20.9% (n=40) were resistant to two or more, including 18.3% (n=35) multidrug-resistant TB (MDR-TB) strains. Monoresistant strains accounted for 6.8% (n=13) of the samples. Genotypic assay of 189 isolates showed 17.5% (n=33) MDR-TB and 5.3% (n=10) isoniazid-monoresistant strains. Genotypic susceptibility results were 99.5% (n=188) concordant and agreed almost perfectly with phenotypic DST (kappa=0.99; 95% confidence interval 0.96-1.01). CONCLUSIONS: The results highlight the burden of TB drug resistance and prove the usefulness of the genotypic DST in Myanmar.
Files in This Item:
T201503930.pdf Download
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Cho, Sang Nae(조상래)
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.