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Lack of ROS1 Gene Rearrangement in Glioblastoma Multiforme

 Sun Min Lim  ;  Junjeong Choi  ;  Jong Hee Chang  ;  Jinyoung Sohn  ;  Kristine Jacobson  ;  Frank Policht  ;  John Schulz  ;  Byoung Chul Cho  ;  Se Hoon Kim 
 PLOS ONE, Vol.10(9) : e0137678, 2015 
Journal Title
Issue Date
Adult ; Aged ; Aged, 80 and over ; Biomarkers/metabolism ; DNA Methylation ; DNA Modification Methylases/genetics ; DNA Repair Enzymes/genetics ; Female ; Gene Rearrangement* ; Glioblastoma/diagnosis ; Glioblastoma/genetics* ; Humans ; Immunohistochemistry ; In Situ Hybridization, Fluorescence ; Isocitrate Dehydrogenase/genetics ; Male ; Middle Aged ; Multivariate Analysis ; Pilot Projects ; Prognosis ; Promoter Regions, Genetic ; Protein-Tyrosine Kinases/genetics* ; Proto-Oncogene Proteins/genetics* ; Tumor Suppressor Proteins/genetics
Glioblastoma multiforme (GBM) is the most aggressive type of brain tumor, and the prognosis remains poor. Rearrangement of ROS1 gene, which was shown to have an oncogenic potential, was previously discovered in GBM cell lines. In this pilot study, we aimed to identify the incidence of ROS1 rearrangement in GBM patient tissues to explore novel biomarkers for therapeutic strategy. Formalin-fixed and paraffin-embedded (FFPE) tissue sections from 109 patients with GBM were screened for ROS1 rearrangement by anti-ROS immunohistochemistry (IHC) and ROS1 break-apart fluorescent in situ hybridization (FISH) assays. O6-methylguanine-DNA methyltransferase (MGMT) gene promoter methylation and Isocitrate dehydrogenase 1 (IDH1) mutation status were also assessed. All samples were interpreted by two experienced pathologists who were blinded to the clinical data. A total of 109 samples were collected and all samples were examined for ROS1 rearrangement by IHC and FISH assays, and none was found to harbor ROS1 rearrangement. MGMT gene methylation was found in 42 (39.2%) cases, and IDH1 mutation was found in 6 (5.5%) cases. In this study, ROS1 rearrangement was not identified in GBM patients, and thus it is difficult to classify ROS1 rearrangement as a novel molecular subset in GBM patients for now.
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1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Se Hoon(김세훈) ORCID logo https://orcid.org/0000-0001-7516-7372
Lim, Sun Min(임선민)
Chang, Jong Hee(장종희)
Cho, Byoung Chul(조병철) ORCID logo https://orcid.org/0000-0002-5562-270X
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