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A distinct role for interleukin-6 as a major mediator of cellular adjustment to an altered culture condition

Authors
 Hwa-Kyung Son  ;  Iha Park  ;  Jue Young Kim  ;  Do Kyeong Kim  ;  Rasika P. Illeperuma  ;  Jung Yoon Bae  ;  Doo Young Lee  ;  Eun-Sang Oh  ;  Da-Woon Jung  ;  Darren R. Williams  ;  Jin Kim 
Citation
 JOURNAL OF CELLULAR BIOCHEMISTRY, Vol.116(11) : 2552-2562, 2015 
Journal Title
 JOURNAL OF CELLULAR BIOCHEMISTRY 
ISSN
 0730-2312 
Issue Date
2015
Keywords
IL-6 ; CULTURE CONDITION ; EPITHELIAL MESENCHYMAL TRANSITION (EMT) ; MESENCHYMAL EPITHELIAL TRANSITION (MET) ; CHOLERA TOXIN
Abstract
Tissue microenvironment adjusts biological properties of different cells by modulating signaling pathways and cell to cell interactions. This study showed that epithelial-mesenchymal transition (EMT)/ mesenchymal-epithelial transition (MET) can be modulated by altering culture conditions. HPV E6/E7-transfected immortalized oral keratinocytes (IHOK) cultured in different media displayed reversible EMT/MET accompanied by changes in cell phenotype, proliferation, gene expression at transcriptional, and translational level, and migratory and invasive activities. Cholera toxin, a major supplement to culture medium, was responsible for inducing the morphological and biological changes of IHOK. Cholera toxin per se induced EMT by triggering the secretion of interleukin 6 (IL-6) from IHOK. We found IL-6 to be a central molecule that modulates the reversibility of EMT based not only on the mRNA level but also on the level of secretion. Taken together, our results demonstrate that IL-6, a cytokine whose transcription is activated by alterations in culture conditions, is a key molecule for regulating reversible EMT/MET. This study will contribute to understand one way of cellular adjustment for surviving in unfamiliar conditions.
Full Text
http://onlinelibrary.wiley.com/doi/10.1002/jcb.25200/abstract
DOI
10.1002/jcb.25200
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소) > 1. Journal Papers
Yonsei Authors
Kim, Jin(김진)
Bae, Jung Yoon(배정윤) ORCID logo https://orcid.org/0000-0001-8342-6987
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/141103
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