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A distinct role for interleukin-6 as a major mediator of cellular adjustment to an altered culture condition

 Hwa-Kyung Son  ;  Iha Park  ;  Jue Young Kim  ;  Do Kyeong Kim  ;  Rasika P. Illeperuma  ;  Jung Yoon Bae  ;  Doo Young Lee  ;  Eun-Sang Oh  ;  Da-Woon Jung  ;  Darren R. Williams  ;  Jin Kim 
 Journal of Cellular Biochemistry, Vol.116(11) : 2552-2562, 2015 
Journal Title
 Journal of Cellular Biochemistry 
Issue Date
Tissue microenvironment adjusts biological properties of different cells by modulating signaling pathways and cell to cell interactions. This study showed that epithelial-mesenchymal transition (EMT)/ mesenchymal-epithelial transition (MET) can be modulated by altering culture conditions. HPV E6/E7-transfected immortalized oral keratinocytes (IHOK) cultured in different media displayed reversible EMT/MET accompanied by changes in cell phenotype, proliferation, gene expression at transcriptional, and translational level, and migratory and invasive activities. Cholera toxin, a major supplement to culture medium, was responsible for inducing the morphological and biological changes of IHOK. Cholera toxin per se induced EMT by triggering the secretion of interleukin 6 (IL-6) from IHOK. We found IL-6 to be a central molecule that modulates the reversibility of EMT based not only on the mRNA level but also on the level of secretion. Taken together, our results demonstrate that IL-6, a cytokine whose transcription is activated by alterations in culture conditions, is a key molecule for regulating reversible EMT/MET. This study will contribute to understand one way of cellular adjustment for surviving in unfamiliar conditions.
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2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소) > 1. Journal Papers
Yonsei Authors
Kim, Jin(김진)
Bae, Jung Yoon(배정윤) ORCID logo https://orcid.org/0000-0001-8342-6987
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