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Effects of In Vitro Osteogenic Induction on In Vivo Tissue Regeneration by Dental Pulp and Periodontal Ligament Stem Cells

DC FieldValueLanguage
dc.contributor.author이효설-
dc.contributor.author전미정-
dc.contributor.author김성오-
dc.contributor.author김승혜-
dc.contributor.author송제선-
dc.contributor.author이제호-
dc.date.accessioned2016-02-04T11:41:32Z-
dc.date.available2016-02-04T11:41:32Z-
dc.date.issued2015-
dc.identifier.issn0099-2399-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/140986-
dc.description.abstractINTRODUCTION: The aim of this study was to determine the effects of in vitro odontogenic/cementogenic differentiation on the in vivo tissue regeneration of dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs). METHODS: DPSCs and PDLSCs were predifferentiated for 0, 4, or 8 days with an odontogenic/cementogenic medium and then transplanted into subcutaneous pockets in immunocompromised mice. The transplants were harvested 9 weeks after transplantation, and the characteristics of the newly formed tissues in vivo were analyzed by histologic staining; examining alkaline phosphate activity; immunohistochemical staining for osteocalcin, dentin sialoprotein, and type XII collagen; and quantitative real-time polymerase chain reaction to analyze the expression patterns of the following genes: RUNX2, OC, DMP1, DSPP, POSTN, CP23, and Col XII. RESULTS: In DPSC transplants, the amount of new tissues was similar in all groups, whereas in predifferentiated transplants the OC and DSPP expression were higher than undifferentiated transplants. Predifferentiated PDLSC transplants generated more hard tissue and expressed higher alkaline phosphatase activity than undifferentiated transplants. In particular, 8-day predifferentiated PDLSC transplants formed tissue closer to the cementum/PDL complex in vivo as confirmed by the higher expression levels of POSTN, CP23, and Col XII. CONCLUSIONS: Although there was no significant increase in tissue-forming ability among DPSCs after predifferentiation, predifferentiated DPSCs generated hard tissue closer to dentin. Also, predifferentiated PDLSCs appeared to be able to generate higher-quality and greater amounts of tissue for dental regeneration than undifferentiated PDLSCs.-
dc.description.statementOfResponsibilityopen-
dc.format.extent1462~1468-
dc.relation.isPartOfJOURNAL OF ENDODONTICS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdolescent-
dc.subject.MESHAdult-
dc.subject.MESHAnimals-
dc.subject.MESHCell Differentiation-
dc.subject.MESHCells, Cultured-
dc.subject.MESHCementogenesis-
dc.subject.MESHChild-
dc.subject.MESHDental Pulp/cytology*-
dc.subject.MESHFemale-
dc.subject.MESHHumans-
dc.subject.MESHMale-
dc.subject.MESHMice-
dc.subject.MESHOdontogenesis-
dc.subject.MESHOsteogenesis*-
dc.subject.MESHPeriodontal Ligament/cytology*-
dc.subject.MESHRegeneration*-
dc.subject.MESHStem Cells/cytology*-
dc.subject.MESHYoung Adult-
dc.titleEffects of In Vitro Osteogenic Induction on In Vivo Tissue Regeneration by Dental Pulp and Periodontal Ligament Stem Cells-
dc.typeArticle-
dc.contributor.collegeCollege of Dentistry (치과대학)-
dc.contributor.departmentDept. of Pediatric Dentistry (소아치과학)-
dc.contributor.googleauthorYoonsun Cha-
dc.contributor.googleauthorMijeong Jeon-
dc.contributor.googleauthorHyo Seol Lee-
dc.contributor.googleauthorSeunghye Kim-
dc.contributor.googleauthorSeong Oh Kim-
dc.contributor.googleauthorJae Ho Lee-
dc.contributor.googleauthorJe Seon Song-
dc.identifier.doi10.1016/j.joen.2015.04.010-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03340-
dc.contributor.localIdA03511-
dc.contributor.localIdA00577-
dc.contributor.localIdA02058-
dc.contributor.localIdA03134-
dc.contributor.localIdA00664-
dc.relation.journalcodeJ01393-
dc.identifier.eissn1878-3554-
dc.identifier.pmid26001856-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0099239915003799-
dc.subject.keywordDental pulp stem cell-
dc.subject.keywordperiodontal ligament stem cell-
dc.subject.keywordpredifferentiation-
dc.subject.keywordregeneration-
dc.contributor.alternativeNameLee, Hyo Seol-
dc.contributor.alternativeNameJeon, Mi Jeong-
dc.contributor.alternativeNameKim, Seong Oh-
dc.contributor.alternativeNameKim, Seung Hye-
dc.contributor.alternativeNameSong, Je Seon-
dc.contributor.alternativeNameLee, Jae Ho-
dc.contributor.affiliatedAuthorLee, Hyo Seol-
dc.contributor.affiliatedAuthorJeon, Mi Jeong-
dc.contributor.affiliatedAuthorKim, Seong Oh-
dc.contributor.affiliatedAuthorSong, Je Seon-
dc.contributor.affiliatedAuthorLee, Jae Ho-
dc.contributor.affiliatedAuthorKim, Seung Hye-
dc.rights.accessRightsnot free-
dc.citation.volume41-
dc.citation.number9-
dc.citation.startPage1462-
dc.citation.endPage1468-
dc.identifier.bibliographicCitationJOURNAL OF ENDODONTICS, Vol.41(9) : 1462-1468, 2015-
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Oral Science Research Center (구강과학연구소) > 1. Journal Papers

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