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Osteocytic Sclerostin Expression in Alveolar Bone in Rats With Diabetes Mellitus and Ligature-Induced Periodontitis

Authors
 Ji Hye Kim  ;  Dong Eun Lee  ;  Gye Hyeong Woo  ;  Jeong Heon Cha  ;  Eun Jung Bak  ;  Yun Jung Yoo 
Citation
 JOURNAL OF PERIODONTOLOGY, Vol.86(8) : 1005-1011, 2015 
Journal Title
 JOURNAL OF PERIODONTOLOGY 
ISSN
 0022-3492 
Issue Date
2015
MeSH
Alveolar Bone Loss/metabolism ; Alveolar Bone Loss/pathology ; Alveolar Process/chemistry* ; Animals ; Bone Matrix/chemistry ; Bone Morphogenetic Proteins/analysis* ; Diabetes Mellitus, Experimental/metabolism* ; Genetic Markers ; Interleukin-1beta/analysis ; Male ; Osteoclasts/chemistry ; Osteoclasts/pathology ; Osteocytes/chemistry* ; Osteocytes/pathology ; Osteogenesis/physiology ; Periodontitis/metabolism* ; Rats ; Rats, Inbred F344 ; Streptozocin ; Time Factors ; Tooth Cervix/pathology ; Tumor Necrosis Factor-alpha/analysis
Keywords
Bone morphogenetic proteins ; diabetes mellitus ; osteogenesis ; periodontitis ; sclerostin protein, rat ; tumor necrosis factor-α
Abstract
BACKGROUND: Osteocytic sclerostin inhibits bone formation, and its expression is stimulated by tumor necrosis factor (TNF)-α. This study investigates sclerostin and TNF-α expression in rats with diabetes mellitus (DM) and periodontitis. METHODS: Rats were divided into control (C), periodontitis (P), and DM + periodontitis (DP) groups. After induction of DM by streptozotocin, periodontitis was induced by ligature. At day 0 (control) and at days 3 and 20 after induction of periodontitis, alveolar bone, osteoclasts, osteoid area, and TNF-α and sclerostin expression were evaluated. RESULTS: The distance between the cemento-enamel junction and the alveolar bone crest of the DP group was longer than that of the P group at day 20 after induction of periodontitis, but the number of osteoclasts was not different. Osteoid area decreased in both the P and DP groups by day 3, but whereas sustained osteoid suppression was observed in the DP group at day 20, osteoid formation was increased in the P group. The number of sclerostin-positive osteocytes increased in both groups at day 3, but the increased number of sclerostin-positive osteocytes was maintained only in the DP group through day 20. The number of TNF-α-positive cells increased more in the DP group than in the P group. CONCLUSIONS: Enhanced alveolar bone loss, suppressed bone formation, and prevalent TNF-α expression were characteristic of the DP group compared with the P group. Suppressed bone formation in the DP group was observed simultaneously with increased sclerostin and TNF-α expression. These results suggest that upregulated osteocytic sclerostin expression in periodontitis accompanied by DM may play a role in suppressed bone formation.
Full Text
http://www.joponline.org/doi/abs/10.1902/jop.2015.150083
DOI
10.1902/jop.2015.150083
Appears in Collections:
5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Bak, Eun-Jung(박은정) ORCID logo https://orcid.org/0000-0002-7976-8594
Yoo, Yun Jung(유윤정) ORCID logo https://orcid.org/0000-0002-0045-9597
Lee, Dong Eun(이동은)
Cha, Jung Heon(차정헌) ORCID logo https://orcid.org/0000-0002-9385-2653
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/140955
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