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Capillary-scale direct measurement of hemoglobin concentration of erythrocytes using photothermal angular light scattering

Authors
 Uihan Kim  ;  Jaewoo Song  ;  Donghak Lee  ;  Suho Ryu  ;  Soocheol Kim  ;  Jaehyun Hwang  ;  Chulmin Joo 
Citation
 BIOSENSORS & BIOELECTRONICS, Vol.74 : 469-475, 2015 
Journal Title
 BIOSENSORS & BIOELECTRONICS 
ISSN
 0956-5663 
Issue Date
2015
MeSH
Adult ; Biosensing Techniques/instrumentation* ; Equipment Design ; Equipment Failure Analysis ; Erythrocytes/metabolism* ; Hemoglobins/metabolism* ; Hot Temperature ; Humans ; Light ; Lighting/instrumentation* ; Male ; Refractometry/instrumentation* ; Reproducibility of Results ; Scattering, Radiation ; Sensitivity and Specificity ; Young Adult
Keywords
Angular light scattering ; Erythrocytes ; Hemoglobin ; Hemoglobin concentration ; Photothermal effect ; Refractive index
Abstract
We present a direct, rapid and chemical-free detection method for hemoglobin concentration ([Hb]), based on photothermal angular light scattering. The iron oxides contained in hemoglobin molecules exhibit high absorption of 532-nm light and generate heat under the illumination of 532-nm light, which subsequently alters the refractive index of blood. We measured this photothermal change in refractive index by employing angular light scattering spectroscopy with the goal of quantifying [Hb] in blood samples. Highly sensitive [Hb] measurement of blood samples was performed by monitoring the shifts in angularly dispersed scattering patterns from the blood-loaded microcapillary tubes. Our system measured [Hb] over the range of 0.35-17.9 g/dL with a detection limit of ~0.12 g/dL. Our sensor was characterized by excellent correlation with a reference hematology analyzer (r>0.96), and yielded a precision of 0.63 g/dL for a blood sample of 9.0 g/dL.
Full Text
http://www.sciencedirect.com/science/article/pii/S0956566315302347
DOI
10.1016/j.bios.2015.06.066
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Song, Jae Woo(송재우) ORCID logo https://orcid.org/0000-0002-1877-5731
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/140851
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