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Stationary-phase induction of vvpS expression by three transcription factors: repression by LeuO and activation by SmcR and CRP

Authors
 Jeong-A. Kim ; Jin Hwan Park ; Kyu-Ho Lee ; Sang-Ho Choi ; Kun-Soo Kim ; Soon-Jung Park ; Hyun-Jung Lee ; Mi-Ae Lee 
Citation
 Molecular Microbiology, Vol.97(2) : 330~346, 2015 
Journal Title
 Molecular Microbiology 
ISSN
 0950-382X 
Issue Date
2015
Abstract
An exoprotease of Vibrio vulnificus, VvpS, exhibits an autolytic function during the stationary phase. To understand how vvpS expression is controlled, the regulators involved in vvpS transcription and their regulatory mechanisms were investigated. LeuO was isolated in a ligand-fishing experiment, and experiments using a leuO-deletion mutant revealed that LeuO represses vvpS transcription. LeuO bound the extended region including LeuO-binding site (LBS)-I and LBS-II. Further screening of additional regulators revealed that SmcR and cyclic adenosine monophosphate-receptor protein (CRP) play activating roles in vvpS transcription. SmcR and CRP bound the regions overlapping LBS-I and -II, respectively. In addition, the LeuO occupancy of LBS-I and LBS-II was competitively exchanged by SmcR and CRP, respectively. To examine the mechanism of stationary-phase induction of vvpS expression, in vivo levels of three transcription factors were monitored. Cellular level of LeuO was maximal at exponential phase, while those of SmcR and CRP were maximal at stationary phase and relatively constant after the early-exponential phase, respectively. Thus, vvpS transcription was not induced during the exponential phase by high cellular content of LeuO. When entering the stationary phase, however, LeuO content was significantly reduced and repression by LeuO was relieved through simultaneous binding of SmcR and CRP to LBS-I and -II, respectively.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/140687
DOI
10.1111/mmi.13028
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Environmental Medical Biology
Yonsei Authors
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Link
 http://onlinelibrary.wiley.com/doi/10.1111/mmi.13028/abstract
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