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O-GlcNAc modification is essential for the regulation of autophagy in Drosophila melanogaster

Authors
 Sujin Park  ;  Yangsin Lee  ;  Jin Won Pak  ;  Hanbyeol Kim  ;  Hyeonjin Choi  ;  Jae-woo Kim  ;  Jürgen Roth  ;  Jin Won Cho 
Citation
 CELLULAR AND MOLECULAR LIFE SCIENCES, Vol.72(16) : 3173-3183, 2015 
Journal Title
 CELLULAR AND MOLECULAR LIFE SCIENCES 
ISSN
 1420-682X 
Issue Date
2015
MeSH
Animals ; Autophagy/physiology* ; Cell Line ; DNA Primers/genetics ; Drosophila Proteins/metabolism ; Drosophila melanogaster/physiology* ; Forkhead Transcription Factors/metabolism ; Immunoblotting ; Immunohistochemistry ; Immunoprecipitation ; Insulin/metabolism* ; Microscopy, Electron, Transmission ; N-Acetylglucosaminyltransferases/metabolism* ; Proto-Oncogene Proteins c-akt/metabolism ; Pyrans ; RNA Interference ; Real-Time Polymerase Chain Reaction ; Signal Transduction/physiology* ; Starvation/metabolism* ; Thiazoles
Keywords
O-GlcNAc modification ; OGT ; Autophagy ; Drosophila melanogaster ; dFOXO
Abstract
O-GlcNAcylation is a dynamic post-translational modification that takes place on ser/thr residues of nucleocytoplasmic proteins. O-GlcNAcylation regulates almost all cellular events as a nutrient sensor, a transcriptional and translational regulator, and a disease-related factor. Although the role of O-GlcNAcylation in insulin signaling and metabolism are well established, the relationship between O-GlcNAcylation and autophagy is largely unknown. Here, we manipulated O-GlcNAcylation in Drosophila and found that it regulates autophagy through Akt/dFOXO signaling. We demonstrate that O-GlcNAcylation and the levels of O-GlcNAc transferase (OGT) are increased during starvation. Furthermore, Atg proteins and autolysosomes are increased in OGT-reduced flies without fasting. Atg proteins and autophagosomes are reduced in OGT-overexpressing flies. Our results suggest that not only autophagy gene expression but also autophagic structures are regulated by OGT through Akt and dFOXO. These data imply that O-GlcNAcylation is important in modulating autophagy as well as insulin signaling in Drosophila.
Full Text
http://link.springer.com/article/10.1007%2Fs00018-015-1889-z
DOI
10.1007/s00018-015-1889-z.
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Jae Woo(김재우) ORCID logo https://orcid.org/0000-0001-5456-9495
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/140656
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