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O-GlcNAc modification is essential for the regulation of autophagy in Drosophila melanogaster

DC Field Value Language
dc.contributor.author김재우-
dc.date.accessioned2016-02-04T11:32:28Z-
dc.date.available2016-02-04T11:32:28Z-
dc.date.issued2015-
dc.identifier.issn1420-682X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/140656-
dc.description.abstractO-GlcNAcylation is a dynamic post-translational modification that takes place on ser/thr residues of nucleocytoplasmic proteins. O-GlcNAcylation regulates almost all cellular events as a nutrient sensor, a transcriptional and translational regulator, and a disease-related factor. Although the role of O-GlcNAcylation in insulin signaling and metabolism are well established, the relationship between O-GlcNAcylation and autophagy is largely unknown. Here, we manipulated O-GlcNAcylation in Drosophila and found that it regulates autophagy through Akt/dFOXO signaling. We demonstrate that O-GlcNAcylation and the levels of O-GlcNAc transferase (OGT) are increased during starvation. Furthermore, Atg proteins and autolysosomes are increased in OGT-reduced flies without fasting. Atg proteins and autophagosomes are reduced in OGT-overexpressing flies. Our results suggest that not only autophagy gene expression but also autophagic structures are regulated by OGT through Akt and dFOXO. These data imply that O-GlcNAcylation is important in modulating autophagy as well as insulin signaling in Drosophila.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfCELLULAR AND MOLECULAR LIFE SCIENCES-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHAutophagy/physiology*-
dc.subject.MESHCell Line-
dc.subject.MESHDNA Primers/genetics-
dc.subject.MESHDrosophila Proteins/metabolism-
dc.subject.MESHDrosophila melanogaster/physiology*-
dc.subject.MESHForkhead Transcription Factors/metabolism-
dc.subject.MESHImmunoblotting-
dc.subject.MESHImmunohistochemistry-
dc.subject.MESHImmunoprecipitation-
dc.subject.MESHInsulin/metabolism*-
dc.subject.MESHMicroscopy, Electron, Transmission-
dc.subject.MESHN-Acetylglucosaminyltransferases/metabolism*-
dc.subject.MESHProto-Oncogene Proteins c-akt/metabolism-
dc.subject.MESHPyrans-
dc.subject.MESHRNA Interference-
dc.subject.MESHReal-Time Polymerase Chain Reaction-
dc.subject.MESHSignal Transduction/physiology*-
dc.subject.MESHStarvation/metabolism*-
dc.subject.MESHThiazoles-
dc.titleO-GlcNAc modification is essential for the regulation of autophagy in Drosophila melanogaster-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Biochemistry & Molecular Biology (생화학,분자생물학)-
dc.contributor.googleauthorSujin Park-
dc.contributor.googleauthorYangsin Lee-
dc.contributor.googleauthorJin Won Pak-
dc.contributor.googleauthorHanbyeol Kim-
dc.contributor.googleauthorHyeonjin Choi-
dc.contributor.googleauthorJae-woo Kim-
dc.contributor.googleauthorJürgen Roth-
dc.contributor.googleauthorJin Won Cho-
dc.identifier.doi10.1007/s00018-015-1889-z-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00865-
dc.relation.journalcodeJ00496-
dc.identifier.eissn1420-9071-
dc.identifier.pmid25840568-
dc.identifier.urlhttp://link.springer.com/article/10.1007%2Fs00018-015-1889-z-
dc.subject.keywordO-GlcNAc modification-
dc.subject.keywordOGT-
dc.subject.keywordAutophagy-
dc.subject.keywordDrosophila melanogaster-
dc.subject.keyworddFOXO-
dc.contributor.alternativeNameKim, Jae Woo-
dc.contributor.affiliatedAuthorKim, Jae Woo-
dc.rights.accessRightsnot free-
dc.citation.volume72-
dc.citation.number16-
dc.citation.startPage3173-
dc.citation.endPage3183-
dc.identifier.bibliographicCitationCELLULAR AND MOLECULAR LIFE SCIENCES, Vol.72(16) : 3173-3183, 2015-
dc.identifier.rimsid30228-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers

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