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Toward angiogenesis of implanted bio-artificial liver using scaffolds with type I collagen and adipose tissue-derived stem cells

 Jae Geun Lee  ;  Seon Young Bak  ;  Ji Hae Nahm  ;  Sang Woo Lee  ;  Seon Ok Min  ;  Kyung Sik Kim 
 Korean Journal of Hepato-Biliary-Pancreatic Surgery, Vol.19(2) : 47-58, 2015 
Journal Title
 Korean Journal of Hepato-Biliary-Pancreatic Surgery (한국간담췌외과학회지) 
Issue Date
Artificial ; Biocompatible materials ; Liver ; Neovascularization ; Physiologic ; Tissue scaffolds
BACKGROUNDS/AIMS: Stem cell therapies for liver disease are being studied by many researchers worldwide, but scientific evidence to demonstrate the endocrinologic effects of implanted cells is insufficient, and it is unknown whether implanted cells can function as liver cells. Achieving angiogenesis, arguably the most important characteristic of the liver, is known to be quite difficult, and no practical attempts have been made to achieve this outcome. We carried out this study to observe the possibility of angiogenesis of implanted bio-artificial liver using scaffolds. METHODS: This study used adipose tissue-derived stem cells that were collected from adult patients with liver diseases with conditions similar to the liver parenchyma. Specifically, microfilaments were used to create an artificial membrane and maintain the structure of an artificial organ. After scratching the stomach surface of severe combined immunocompromised (SCID) mice (n=4), artificial scaffolds with adipose tissue-derived stem cells and type I collagen were implanted. Expression levels of angiogenesis markers including vascular endothelial growth factor (VEGF), CD34, and CD105 were immunohistochemically assessed after 30 days. RESULTS: Grossly, the artificial scaffolds showed adhesion to the stomach and surrounding organs; however, there was no evidence of angiogenesis within the scaffolds; and VEGF, CD34, and CD105 expressions were not detected after 30 days. CONCLUSIONS: Although implantation of cells into artificial scaffolds did not facilitate angiogenesis, the artificial scaffolds made with type I collagen helped maintain implanted cells, and surrounding tissue reactions were rare. Our findings indicate that type I collagen artificial scaffolds can be considered as a possible implantable biomaterial.
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1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Sik(김경식) ORCID logo https://orcid.org/0000-0001-9498-284X
Nahm, Ji Hae(남지해) ORCID logo https://orcid.org/0000-0003-0902-866X
Min, Seon Ok(민선옥)
Bak, Seon Young(박선영)
Lee, Sang Woo(이상우)
Lee, Jae Geun(이재근) ORCID logo https://orcid.org/0000-0002-6722-0257
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