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Toward angiogenesis of implanted bio-artificial liver using scaffolds with type I collagen and adipose tissue-derived stem cells

Authors
 Jae Geun Lee  ;  Seon Young Bak  ;  Ji Hae Nahm  ;  Sang Woo Lee  ;  Seon Ok Min  ;  Kyung Sik Kim 
Citation
 Korean Journal of Hepato-Biliary-Pancreatic Surgery, Vol.19(2) : 47-58, 2015 
Journal Title
 Korean Journal of Hepato-Biliary-Pancreatic Surgery  (한국간담췌외과학회지) 
ISSN
 1738-6349 
Issue Date
2015
Abstract
BACKGROUNDS/AIMS: Stem cell therapies for liver disease are being studied by many researchers worldwide, but scientific evidence to demonstrate the endocrinologic effects of implanted cells is insufficient, and it is unknown whether implanted cells can function as liver cells. Achieving angiogenesis, arguably the most important characteristic of the liver, is known to be quite difficult, and no practical attempts have been made to achieve this outcome. We carried out this study to observe the possibility of angiogenesis of implanted bio-artificial liver using scaffolds. METHODS: This study used adipose tissue-derived stem cells that were collected from adult patients with liver diseases with conditions similar to the liver parenchyma. Specifically, microfilaments were used to create an artificial membrane and maintain the structure of an artificial organ. After scratching the stomach surface of severe combined immunocompromised (SCID) mice (n=4), artificial scaffolds with adipose tissue-derived stem cells and type I collagen were implanted. Expression levels of angiogenesis markers including vascular endothelial growth factor (VEGF), CD34, and CD105 were immunohistochemically assessed after 30 days. RESULTS: Grossly, the artificial scaffolds showed adhesion to the stomach and surrounding organs; however, there was no evidence of angiogenesis within the scaffolds; and VEGF, CD34, and CD105 expressions were not detected after 30 days. CONCLUSIONS: Although implantation of cells into artificial scaffolds did not facilitate angiogenesis, the artificial scaffolds made with type I collagen helped maintain implanted cells, and surrounding tissue reactions were rare. Our findings indicate that type I collagen artificial scaffolds can be considered as a possible implantable biomaterial.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/140441
Files in This Item:
T201502090.pdf Download
DOI
10.14701/kjhbps.2015.19.2.47
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원)
Yonsei Authors
김경식(Kim, Kyung Sik)
남지해(Nahm, Ji Hae) ORCID logo https://orcid.org/0000-0003-0902-866X
민선옥(Min, Seon Ok)
박선영(Bak, Seon Young)
이상우(Lee, Sang Woo)
이재근(Lee, Jae Geun)
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