Cited 18 times in
PSA-NCAM-negative neural crest cells emerging during neural induction of pluripotent stem cells cause mesodermal tumors and unwanted grafts
DC Field | Value | Language |
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dc.contributor.author | 김동욱 | - |
dc.contributor.author | 김한수 | - |
dc.contributor.author | 박상현 | - |
dc.contributor.author | 박철용 | - |
dc.contributor.author | 유정은 | - |
dc.contributor.author | 이동진 | - |
dc.contributor.author | 이재석 | - |
dc.contributor.author | 이준원 | - |
dc.contributor.author | 지은현 | - |
dc.date.accessioned | 2016-02-04T11:26:37Z | - |
dc.date.available | 2016-02-04T11:26:37Z | - |
dc.date.issued | 2015 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/140435 | - |
dc.description.abstract | Tumorigenic potential of human pluripotent stem cells (hPSCs) is an important issue in clinical applications. Despite many efforts, PSC-derived neural precursor cells (NPCs) have repeatedly induced tumors in animal models even though pluripotent cells were not detected. We found that polysialic acid-neural cell adhesion molecule (PSA-NCAM)(-) cells among the early NPCs caused tumors, whereas PSA-NCAM(+) cells were nontumorigenic. Molecular profiling, global gene analysis, and multilineage differentiation of PSA-NCAM(-) cells confirm that they are multipotent neural crest stem cells (NCSCs) that could differentiate into both ectodermal and mesodermal lineages. Transplantation of PSA-NCAM(-) cells in a gradient manner mixed with PSA-NCAM(+) cells proportionally increased mesodermal tumor formation and unwanted grafts such as PERIPHERIN(+) cells or pigmented cells in the rat brain. Therefore, we suggest that NCSCs are a critical target for tumor prevention in hPSC-derived NPCs, and removal of PSA-NCAM(-) cells eliminates the tumorigenic potential originating from NCSCs after transplantation. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | STEM CELL REPORTS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Differentiation | - |
dc.subject.MESH | Cell Lineage | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Ectoderm/cytology | - |
dc.subject.MESH | Ectoderm/metabolism | - |
dc.subject.MESH | Human Embryonic Stem Cells/cytology | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunohistochemistry | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mesoderm/cytology | - |
dc.subject.MESH | Mesoderm/metabolism | - |
dc.subject.MESH | Neoplasms/etiology | - |
dc.subject.MESH | Neoplasms/metabolism | - |
dc.subject.MESH | Neural Cell Adhesion Molecule L1/genetics | - |
dc.subject.MESH | Neural Cell Adhesion Molecule L1/metabolism* | - |
dc.subject.MESH | Neural Crest/cytology | - |
dc.subject.MESH | Neural Crest/metabolism* | - |
dc.subject.MESH | Neural Crest/transplantation | - |
dc.subject.MESH | Peripherins/metabolism | - |
dc.subject.MESH | Pluripotent Stem Cells/cytology* | - |
dc.subject.MESH | Pluripotent Stem Cells/metabolism | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Sialic Acids/genetics | - |
dc.subject.MESH | Sialic Acids/metabolism* | - |
dc.subject.MESH | Transcriptome | - |
dc.title | PSA-NCAM-negative neural crest cells emerging during neural induction of pluripotent stem cells cause mesodermal tumors and unwanted grafts | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Physiology (생리학) | - |
dc.contributor.googleauthor | Dongjin R. Lee | - |
dc.contributor.googleauthor | Jeong-Eun Yoo | - |
dc.contributor.googleauthor | Jae Souk Lee | - |
dc.contributor.googleauthor | Sanghyun Park | - |
dc.contributor.googleauthor | Junwon Lee | - |
dc.contributor.googleauthor | Chul-Yong Park | - |
dc.contributor.googleauthor | Eunhyun Ji | - |
dc.contributor.googleauthor | Han-Soo Kim | - |
dc.contributor.googleauthor | Dong-Youn Hwang | - |
dc.contributor.googleauthor | Dae-Sung Kim | - |
dc.contributor.googleauthor | Dong-Wook Kim | - |
dc.identifier.doi | 10.1016/j.stemcr.2015.04.002 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01100 | - |
dc.contributor.localId | A01719 | - |
dc.contributor.localId | A02734 | - |
dc.contributor.localId | A03074 | - |
dc.contributor.localId | A03179 | - |
dc.contributor.localId | A03969 | - |
dc.contributor.localId | A02505 | - |
dc.contributor.localId | A01491 | - |
dc.contributor.localId | A00406 | - |
dc.relation.journalcode | J02679 | - |
dc.identifier.eissn | 2213-6711 | - |
dc.identifier.pmid | 25937368 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S2213671115001022 | - |
dc.contributor.alternativeName | Kim, Dong Wook | - |
dc.contributor.alternativeName | Kim, Han Soo | - |
dc.contributor.alternativeName | Park, Sang Hyun | - |
dc.contributor.alternativeName | Park, Chul Yong | - |
dc.contributor.alternativeName | Yoo, Jeong Eun | - |
dc.contributor.alternativeName | Lee, Dongjin R. | - |
dc.contributor.alternativeName | Lee, Jae Souk | - |
dc.contributor.alternativeName | Lee, Jun Won | - |
dc.contributor.alternativeName | Ji, Eun Hyun | - |
dc.contributor.affiliatedAuthor | Kim, Han Soo | - |
dc.contributor.affiliatedAuthor | Park, Chul Yong | - |
dc.contributor.affiliatedAuthor | Lee, Dongjin R. | - |
dc.contributor.affiliatedAuthor | Lee, Jae Souk | - |
dc.contributor.affiliatedAuthor | Lee, Jun Won | - |
dc.contributor.affiliatedAuthor | Ji, Eun Hyun | - |
dc.contributor.affiliatedAuthor | Yoo, Jeong Eun | - |
dc.contributor.affiliatedAuthor | Park, Sang Hyun | - |
dc.contributor.affiliatedAuthor | Kim, Dong Wook | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 4 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 821 | - |
dc.citation.endPage | 834 | - |
dc.identifier.bibliographicCitation | STEM CELL REPORTS, Vol.4(5) : 821-834, 2015 | - |
dc.identifier.rimsid | 52009 | - |
dc.type.rims | ART | - |
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