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조혈모세포로부터 적혈구 분화에 사용되는 무혈청 배지의 성능비교

Other Titles
 Comparison of Serum-Free Media in RBC Differentiation from Human Hematopoietic Stem Cells 
Authors
 김지연  ;  김신영  ;  전유라  ;  최용욱  ;  김현옥 
Citation
 Korean Journal of Blood Transfusion (대한수혈학회지), Vol.26(1) : 18-25, 2015 
Journal Title
 Korean Journal of Blood Transfusion  (대한수혈학회지) 
ISSN
 1226-9336 
Issue Date
2015
Abstract
Background: Research on RBC production from hematopoietic stem cells has been conducted competitively in many countries. However those were in vitro successes and many hurdles still remain for large scale transfusable RBC production from stem cells. A need for large volume of culture media is a crucial factor for culture condition which researchers must overcome. In this study, we evaluated the efficiency of two commercial serum-free media, StemPro®-34 SFM and Stemline II hematopoietic stem cell expansion medium, in RBC differentiation from cord derived stem cells. Methods: We cultured cord derived CD34+ cells in vitro and evaluated over the periods of 7 days, 14 days, 17 days and 21 days in culture for expanded cell count, cell morphology and differential count using the Wright Giemsa stain. Results: Cell expansion and RBC differentiation developed rapidly in Stemline media compared to StemPro media. Enucleated RBCs were observed at 10∼14 culture days and orthochromatic erythroblasts were shown up to 50 % among culture cells at 17 days in Stemline media. The enucleated RBCs were observed at 17 days in StemPro Media. Although the erythroblasts in StemPro media are slow at differentiation, they maintain continuous expansion up to 21 days. Conclusion: In Stemline media, the expansion and differentiation to mature RBCs are processed much faster, but the cell condition slows down after 17 days. In the RBC production aspects, Stemline media is better than StemPro media as a rapid differentiation because it reduces the cost due to in vitro short culture duration.
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T201501926.pdf Download
DOI
10.17945/kjbt.2015.26.1.18
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
김신영(Kim, Sin Young) ORCID logo https://orcid.org/0000-0002-2609-8945
김현옥(Kim, Hyun Ok) ORCID logo https://orcid.org/0000-0002-4964-1963
전유라(Jeon, You La)
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/140367
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