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Rapid Reticulin Fiber Staining Method is Helpful for the Diagnosis of Pituitary Adenoma in Frozen Section

Authors
 Songmi Noh  ;  Sun Ho Kim  ;  Nam Hoon Cho  ;  Se Hoon Kim 
Citation
 Endocrine Pathology, Vol.26(2) : 178-184, 2015 
Journal Title
 Endocrine Pathology 
ISSN
 1046-3976 
Issue Date
2015
MeSH
Adenoma/diagnosis* ; Adenoma/metabolism ; Adenoma/pathology ; Adenoma/surgery ; Adolescent ; Adult ; Aged ; Female ; Frozen Sections* ; Humans ; Intraoperative Period ; Male ; Middle Aged ; Neoplasm Invasiveness ; Pituitary Neoplasms/diagnosis* ; Pituitary Neoplasms/metabolism ; Pituitary Neoplasms/pathology ; Pituitary Neoplasms/surgery ; Predictive Value of Tests ; Reticulin/metabolism* ; Silver Staining* ; Time Factors ; Young Adult
Keywords
Pituitary adenoma ; Frozen sections ; Reticulin fiber ; Silver impregnation methods
Abstract
Approximately 90% of neoplasms found in the sellar region are adenoma of the pituitary gland. The use of frozen sections for the diagnosis of pituitary adenomas has an accuracy of 90% and is useful in evaluating complete tumor removal. However, it is sometimes difficult to diagnose pituitary adenomas using frozen sections because of the small sample size and marked artifact, and the contiguity of the pituitary adenoma with normal pituitary gland tissue. In this study, we evaluated the use of our modified reticulin stain to make correct decision in frozen section with reduced stain time and investigated the objective diagnostic criteria of pituitary adenoma with reticulin stain. We used Gomori's silver impregnation methods to stain reticulin fibers in frozen pituitary gland sections of 36 samples from 24 patients. We modified the conventional staining method by reducing the overall staining time. We diagnosed pituitary lesion according to our interpretation criteria and compared the results to those of the conventional method and findings of hematoxylin and eosin-stained slides. Reticulin fiber staining of normal adenohypophysis outlines the supporting stroma around the blood vessels and shows regular of the gland meshwork interconnecting the capillaries. In contrast, reticulin fiber staining of the adenomatous tissue shows loss of meshwork or frequent fragmentation. Our modified reticulin stain is more rapid than the established method and shows similar levels of accuracy. Independent evaluation by two pathologists showed discrepancies in diagnosis in four out of 36 cases with modified reticulin stain. Our rapid modified reticulin staining method for frozen sections may be useful as a diagnostic tool for pituitary adenomas and can complement routine hematoxylin and eosin staining.
Full Text
http://link.springer.com/article/10.1007%2Fs12022-015-9370-y
DOI
10.1007/s12022-015-9370-y
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sun Ho(김선호) ORCID logo https://orcid.org/0000-0003-0970-3848
Kim, Se Hoon(김세훈) ORCID logo https://orcid.org/0000-0001-7516-7372
Cho, Nam Hoon(조남훈) ORCID logo https://orcid.org/0000-0002-0045-6441
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/140283
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