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ASK1 modulates the expression of microRNA Let7A in microglia under high glucose in vitro condition

Authors
 Juhyun Song  ;  Jong Eun Lee 
Citation
 FRONTIERS IN CELLULAR NEUROSCIENCE, Vol.9 : 198, 2015 
Journal Title
 FRONTIERS IN CELLULAR NEUROSCIENCE 
Issue Date
2015
Keywords
Microglia BV2 ; N-Myc ; apoptosis signal regulating kinase 1 (ASK1) ; c-Myc ; cytokine ; microRNA-Let7A (miR-Let7A)
Abstract
Hyperglycemia results in oxidative stress and leads to neuronal apoptosis in the brain. Diabetes studies show that microglia participate in the progression of neuropathogenesis through their involvement in inflammation in vivo and in vitro. In high-glucose-induced inflammation, apoptosis signal regulating kinase 1 (ASK1) triggers the release of apoptosis cytokines and apoptotic gene expression. MicroRNA-Let7A (miR-Let7A) is reported to be a regulator of inflammation. In the present study, we investigated whether miR-Let7A regulates the function of microglia by controlling ASK1 in response to high-glucose-induced oxidative stress. We performed reverse transcription (RT) polymerase chain reaction, Taqman assay, real-time polymerase chain reaction, and immunocytochemistry to confirm the alteration of microglia function. Our results show that miR-Let7A is associated with the activation of ASK1 and the expression of anti-inflammatory cytokine (interleukin (IL)-10) and Mycs (c-Myc and N-Myc). Thus, the relationship between Let-7A and ASK1 could be a novel target for enhancing the beneficial function of microglia in central nervous system (CNS) disorders.
Files in This Item:
T201501694.pdf Download
DOI
10.3389/fncel.2015.00198
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Song, Ju Hyun(송주현)
Lee, Jong Eun(이종은) ORCID logo https://orcid.org/0000-0001-6203-7413
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/140254
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