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In vitro evaluation of allergen potencies of commercial house dust mite sublingual immunotherapy reagents

DC FieldValueLanguage
dc.contributor.author박경희-
dc.contributor.author박중원-
dc.contributor.author박혜정-
dc.contributor.author이재현-
dc.contributor.author정경용-
dc.contributor.author최수영-
dc.date.accessioned2016-02-04T11:20:46Z-
dc.date.available2016-02-04T11:20:46Z-
dc.date.issued2015-
dc.identifier.issn2092-7355-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/140220-
dc.description.abstractPURPOSE: The clinical efficacy of allergen-immunotherapy is known to be dose dependent. However, optimal maintenance dosage has not yet been determined for sublingual immunotherapy (SLIT). Furthermore, since companies adopt their own units for expression of allergenicity, the allergen concentrations of individual reagents cannot be compared easily. We sought to measure and compare the allergenicities of 3 commercially available house dust mite (HDM) SLIT regents and a subcutaneous immunotherapy reagent. METHODS: We measured the HDM allergenic potency of the maintenance dosages of three SLIT reagents: Staloral® (300 index of reactivity [IR] /mL, recommended maintenance dosage [MD]: 120 IR), SLITone® (1,000 standard therapeutic unit [STU]/mL, recommended MD: 200 STU), Wolwopharma® (100 µg/mL, recommended MD: 20 µg), and subcutaneous immunotherapy regents of Hollister-Stier (10,000 allergy unit [AU] /mL). The allergenic potency was assessed by measuring the total protein concentrations, mite group 1 and 2 allergens using 2-site ELISA, and an inhibition test against IgE specific to Dermatophagoides farinae and Dermatophagoides pteronyssinus. RESULTS: The protein content of the Wolwopharma® reagent was 1.5-261.4 times higher than that of the other 2 SLIT reagents. The concentration of group 1 major allergens in Staloral® (132.03 µg/mL) was 33- to 44.5-fold higher than in SLITone® (4.00 µg/mL) and Wolwopharma® (2.97 µg/mL). The concentration of group 2 major allergen was also 8.9- to 10.5-fold higher in Staloral® (15.7 µg/mL) than in SLITone® (1.8 µg/mL) or Wolwopharma® (1.5 µg/mL). An ELISA inhibition study against HDM-specific IgE showed that the allergen potency of Staloral® reagent is 8.5-fold and 21-fold higher than that of SLITone® or Wolwopharma®, respectively. The differences between the maintenance dosages are further exaggerated by the differences in the recommended volumes of SLIT reagents. CONCLUSIONS: The allergen potencies of commercially available HDM SLIT reagents are markedly different. Consensus regarding the optimal allergen concentration for SLIT reagents used to treat HDM respiratory allergies is needed.-
dc.description.statementOfResponsibilityopen-
dc.format.extent124~129-
dc.relation.isPartOfALLERGY ASTHMA & IMMUNOLOGY RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleIn vitro evaluation of allergen potencies of commercial house dust mite sublingual immunotherapy reagents-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorKyung Hee Park-
dc.contributor.googleauthorMina Son-
dc.contributor.googleauthorSoo-Young Choi-
dc.contributor.googleauthorHey Jung Park-
dc.contributor.googleauthorJae-Hyun Lee-
dc.contributor.googleauthorKyoung Yong Jeong-
dc.contributor.googleauthorJoo-Shil Lee-
dc.contributor.googleauthorJung-Won Park-
dc.identifier.doi10.4168/aair.2015.7.2.124-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01427-
dc.contributor.localIdA01681-
dc.contributor.localIdA01769-
dc.contributor.localIdA03086-
dc.contributor.localIdA03572-
dc.contributor.localIdA04093-
dc.relation.journalcodeJ00064-
dc.identifier.eissn2092-7363-
dc.identifier.pmid25729619-
dc.subject.keywordHouse dust mite-
dc.subject.keywordstandardization-
dc.subject.keywordsublingual immunotherapy-
dc.contributor.alternativeNamePark, Kyung Hee-
dc.contributor.alternativeNamePark, Jung Won-
dc.contributor.alternativeNamePark, Hye Jung-
dc.contributor.alternativeNameLee, Jae Hyun-
dc.contributor.alternativeNameJeong, Kyoung Yong-
dc.contributor.alternativeNameChoi, Soo Young-
dc.contributor.affiliatedAuthorPark, Kyung Hee-
dc.contributor.affiliatedAuthorPark, Jung Won-
dc.contributor.affiliatedAuthorPark, Hye Jung-
dc.contributor.affiliatedAuthorLee, Jae Hyun-
dc.contributor.affiliatedAuthorJeong, Kyoung Yong-
dc.contributor.affiliatedAuthorChoi, Soo Young-
dc.rights.accessRightsfree-
dc.citation.volume7-
dc.citation.number2-
dc.citation.startPage124-
dc.citation.endPage129-
dc.identifier.bibliographicCitationALLERGY ASTHMA & IMMUNOLOGY RESEARCH, Vol.7(2) : 124-129, 2015-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Institute of Allergy (알레르기연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers

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