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Role of AcsR in expression of the acetyl-CoA synthetase gene in Vibrio vulnificus

 Min Jung Kim  ;  Juri Kim  ;  Hye Yeon Lee  ;  Hyeon Jin Noh  ;  Kyu-Ho Lee  ;  Soon-Jung Park 
 BMC MICROBIOLOGY, Vol.15 : 86, 2015 
Journal Title
Issue Date
Acetate-CoA Ligase/metabolism* ; Artificial Gene Fusion ; Culture Media/chemistry ; DNA, Bacterial/metabolism ; Electrophoretic Mobility Shift Assay ; Gene Deletion ; Gene Expression Profiling ; Gene Expression Regulation, Bacterial* ; Genes, Reporter ; Genetic Complementation Test ; Luciferases/analysis ; Luciferases/genetics ; Protein Binding ; Transcription Factors/deficiency ; Transcription Factors/metabolism* ; Vibrio vulnificus/enzymology* ; Vibrio vulnificus/growth & development
Vibrio vulnificus ; Acetate metabolism ; Acetyl-CoA synthetase ; AcsR ; VarS/VarA
BACKGROUND: VarS/VarA is one of the global factors regulating diverse aspects of the metabolism and virulence of bacteria including pathogenic Vibrio spp. An experiment to identify the VarS/VarA-regulon in V. vulnificus revealed that a putative LuxR-type transcriptional regulator was down-regulated in ΔvarA mutant. To investigate the roles of this regulatory cascade, the target gene regulated by a LuxR-regulator was identified and its expression was characterized. RESULTS: Transcriptomic analysis of the mutant deficient in this LuxR-type regulator showed that the acsA gene encoding acetyl-CoA synthetase was down-regulated. Thus, this regulator was named AcsR for "regulator of acetyl-CoA synthetase". A putative histidine kinase gene, acsS, was located five ORFs downstream of the acsR gene. Expression of an acsA::luxAB transcriptional fusion was decreased in both ΔacsR and ΔacsS mutants. Similar to a ΔacsA mutant, strains carrying deletions either in acsR or acsS grew slowly than wild type in a minimal medium with acetate as a sole carbon source. Growth defect of the ΔacsR strain in acetate-minimal medium was restored by complementation. To investigate if AcsR directly regulates acsA expression, in vitro-gel shift assays were performed using the recombinant AcsR and the regulatory region of the acsA gene, showing that AcsR specifically bound the upstream region of the acsA ORF. CONCLUSION: This study indicates that the VarS/VarA system plays a role in V. vulnificus metabolism via regulating AcsR, which in turn controls acetate metabolism by activating the transcription of the acetyl-CoA synthetase gene.
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1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Min Jung(김민정)
Kim, Ju Ri(김주리) ORCID logo https://orcid.org/0000-0001-8270-7584
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
Lee, Hye Yeon(이혜연)
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