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HOXB5 Promotes the Proliferation and Invasion of Breast Cancer Cells

Authors
 Ji-Yeon Lee  ;  Ho Hur  ;  Hyo Jung Yun  ;  Yeejeong Kim  ;  Seoyeon Yang  ;  Seung Il Kim  ;  Myoung Hee Kim 
Citation
 INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES, Vol.11(6) : 701-711, 2015 
Journal Title
 INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 
Issue Date
2015
MeSH
Breast Neoplasms/genetics* ; Breast Neoplasms/metabolism ; Breast Neoplasms/pathology ; Cell Line, Tumor ; Cell Proliferation/genetics ; Female ; Gene Expression Regulation, Neoplastic ; Gene Knockdown Techniques ; Homeodomain Proteins/genetics ; Homeodomain Proteins/metabolism ; Homeodomain Proteins/physiology* ; Humans ; Immunohistochemistry ; Neoplasm Invasiveness/genetics
Keywords
ER-positive ; HOXB5 ; aberrant expression ; breast cancer ; epithelial-mesenchymal transition
Abstract
HOX transcription factors play an important role in determining body patterning and cell fate during embryogenesis. Accumulating evidence has shown that these genes act as positive and/or negative modulators in many types of cancer, including breast cancer, in a tissue-specific manner. We have previously reported that HOXB5 is aberrantly overexpressed in breast cancer tissues and cell lines. Here, we investigated the biological roles and clinical relevance of HOXB5 in breast cancer. Immunohistochemical analysis of HOXB5 on tissue microarray (TMA) including 34 normal and 67 breast cancer specimens revealed that HOXB5 was highly expressed in cancer tissues, particularly from estrogen receptor (ER)-positive breast cancer patients. An online survival analysis confirmed the correlation between HOXB5 expression and poor distant metastasis-free survival in ER-positive, but not in ER-negative, breast cancer. In vitro studies indicated that HOXB5 silencing in ER-positive cells significantly decreased cell proliferation and anchorage-independent cell growth. In contrast, overexpression of HOXB5 displayed EMT characteristics with a greater invasive ability, higher cell proliferation and colony formation in soft agar. HOXB5 knockdown or overexpression led to changes in the expression levels of RET, ERBB2, and EGFR, but not of ESR1. In conclusion, we suggest that HOXB5 acts as a positive modulator most likely by promoting cell proliferative response and invasiveness in ER-positive breast cancer. These results would help predict prognosis of breast cancer and identify a new valuable therapeutic target
Files in This Item:
T201501382.pdf Download
DOI
10.7150/ijbs.11431
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Kim, Myoung Hee(김명희) ORCID logo https://orcid.org/0000-0001-5652-1452
Kim, Seung Il(김승일)
Lee, Ji Yeon(이지연) ORCID logo https://orcid.org/0000-0002-0670-3095
Hur, Ho(허호)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/140087
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