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Circulating microRNAs as potential biomarkers for endometriosis

 SiHyun Cho  ;  Levent Mutlu  ;  Olga Grechukhina  ;  Hugh S. Taylor 
 FERTILITY AND STERILITY, Vol.103(5) : 1252-1260, 2015 
Journal Title
Issue Date
Adolescent ; Adult ; Area Under Curve ; Case-Control Studies ; Cell Proliferation ; Endometriosis/blood ; Endometriosis/diagnosis* ; Endometriosis/genetics* ; Female ; Genetic Markers ; Hospitals, University ; Humans ; Logistic Models ; MicroRNAs/blood ; MicroRNAs/genetics* ; Middle Aged ; Predictive Value of Tests ; ROC Curve ; Real-Time Polymerase Chain Reaction ; Reverse Transcriptase Polymerase Chain Reaction ; Young Adult
Biomarker ; circulating miRNAs ; diagnosis ; endometriosis ; let-7
OBJECTIVE: To evaluate whether microRNAs (miRNAs) associated with endometriosis are detectable in the circulation and could serve as potential noninvasive biomarkers for endometriosis. DESIGN: Case-control study. SETTING: University hospital. PATIENT(S): Twenty-four women with endometriosis and 24 women without the disease (controls). INTERVENTION(S): Serum samples collected from women undergoing laparoscopy for endometriosis and other benign gynecologic disease. MAIN OUTCOME MEASURE(S): Total RNA extracted from serum and quantitative reverse-transcription polymerase chain reaction to determine levels of miRNA let-7a-f and miR-135a,b. RESULT(S): The levels of circulating let-7b and miR-135a were statistically significantly decreased in women with endometriosis compared with controls, and let-7d and 7f showed a trend toward down-regulation. Let-7b expression strongly correlated with serum CA-125 levels and showed the highest area under the curve of 0.691. When the patients were analyzed according to phase of the menstrual cycle, the expression of let-7b, 7c, 7d, and 7e was statistically significantly lower in the women with endometriosis during the proliferative phase. Using a logistic regression model, we evaluated the diagnostic power of differently expressed miRNAs; the combination of let-7b, let-7d, and let-7f during the proliferative phase yielded the highest area under the curve value of 0.929 in discriminating endometriosis from controls. CONCLUSION(S): Several circulating miRNAs are differentially expressed in the sera of women with endometriosis compared with controls. The combination of serum let-7b, 7d, and 7f levels during the proliferative phase may serve as a diagnostic marker for endometriosis.
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1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
Yonsei Authors
Cho, Si Hyun(조시현) ORCID logo https://orcid.org/0000-0003-2718-6645
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