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Bacterial PAMPs and Allergens Trigger Increase in [Ca(2+)]i-induced Cytokine Expression in Human PDL Fibroblasts

Authors
 Ga Yeon Son  ;  Dong Min Shin  ;  Jeong Hee Hong 
Citation
 KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY, Vol.19(3) : 291-297, 2015 
Journal Title
 KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY 
ISSN
 1226-4512 
Issue Date
2015
Keywords
Calcium signaling ; Cytokines ; Inflammation ; Interleukins ; Periodontal ligament
Abstract
An oral environment is constantly exposed to environmental factors and microorganisms. The periodontal ligament (PDL) fibroblasts within this environment are subject to bacterial infection and allergic reaction. However, how these condition affect PDL fibroblasts has yet to be elucidated. PDL fibroblasts were isolated from healthy donors. We examined using reverse transcription-polymerase chain reaction and measuring the intracellular Ca(2+) concentration ([Ca(2+)]i). This study investigated the receptors activated by exogenous bacterial pathogens (Lipopolysaccharide and peptidoglycan) and allergens (German cockroach extract and house dust mite) as well as these pathogenic mediators-induced effects on the intracellular Ca(2+) signaling in human PDL fibroblasts. Moreover, we evaluated the expression of pro-inflammatory cytokines (interleukin (IL)-1β, IL-6, and IL-8) and bone remodeling mediators (receptor activator of NF-κB ligand and osteoprotegerin) and intracellular Ca(2+)-involved effect. Bacterial pathogens and allergic mediators induced increased expression of pro-inflammatory cytokines, and these results are dependent on intracellular Ca(2+). However, bacterial pathogens and allergic mediators did not lead to increased expression of bone remodeling mediators, except lipopolysaccharide-induced effect on receptor activator of NF-κB ligand expression. These experiments provide evidence that a pathogens and allergens-induced increase in [Ca(2+)]i affects the inflammatory response in human PDL fibroblasts.
Files in This Item:
T201501332.pdf Download
DOI
10.4196/kjpp.2015.19.3.291
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Son, Ga Yeon(손가연)
Shin, Dong Min(신동민) ORCID logo https://orcid.org/0000-0001-6042-0435
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/140037
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