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Crystal structure of CagL from Helicobacter pylori K74 strain

Authors
 Jin Myung Choi  ;  Yun Hui Choi  ;  Muddenahalli Srinivasa Sudhanva  ;  Sundaravinayagam Devakumar  ;  Kun Ho Lee  ;  Jeong-Heon Cha  ;  Sung Haeng Lee 
Citation
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.460(4) : 964-970, 2015 
Journal Title
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 
ISSN
 0006-291X 
Issue Date
2015
MeSH
Amino Acid Sequence ; Bacterial Proteins/chemistry* ; Bacterial Proteins/metabolism ; Crystallization ; Crystallography, X-Ray ; Helicobacter pylori/chemistry* ; Helicobacter pylori/metabolism ; Models, Molecular ; Molecular Sequence Data ; Protein Binding ; Sequence Homology, Amino Acid
Keywords
CagL ; Helicobacter pylori ; Protein structure ; RGD domain ; T4SS
Abstract
Helicobacter pylori (Hp) CagL is a component of the type IV secretion system (T4SS) and interacts with integrin in host cells through its flexible RGD domain to translocate CagA. Differences in CagL amino acid polymorphisms between Western and East-Asian Hps are correlated with clinical outcome. CagL of East-Asian clinical Hp isolate K74 (CagL(K74)) contains multiple residue variations upstream of RGD motif and has different integrin binding affinities compared to those of CagL from Western Hp 26695. Here, we report the crystal structure of CagL(K74). The structure displayed a six-helix bundle including two short α-helices, and the RGD motif was found in the long rigid α2 helix flanked by the conserved protease-sensitive and RGD-helper sequences, as observed in CagL(26695). However, two additional salt bridges were found between the helices compared with the CagL(26695) structure, suggesting that the putative flexible region harboring the RGD motif may be more stable in this CagL variant.
Full Text
http://www.sciencedirect.com/science/article/pii/S0006291X15006014
DOI
10.1016/j.bbrc.2015.03.135
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Cha, Jung Heon(차정헌) ORCID logo https://orcid.org/0000-0002-9385-2653
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/139994
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