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The effects of hydrogen gas inhalation during ex vivo lung perfusion on donor lungs obtained after cardiac death

Authors
 Seokjin Haam  ;  Sungsoo Lee  ;  Hyo Chae Paik  ;  Moo Suk Park  ;  Joo Han Song  ;  Beom Jin Lim  ;  Atsunori Nakao 
Citation
 European Journal of Cardio-Thoracic Surgery, Vol.48(4) : 542-547, 2015 
Journal Title
 European Journal of Cardio-Thoracic Surgery 
ISSN
 1010-7940 
Issue Date
2015
Abstract
OBJECTIVES: Lung transplantation is a well-established treatment of end-stage lung disease; however, it is limited by a shortage of donor lungs. To overcome this problem, donation after cardiac death (DCD) and ex vivo lung perfusion (EVLP) are being widely investigated. In this study, the effect of hydrogen gas, a known antioxidant, was investigated on a DCD lung model during EVLP. METHODS: Ten pigs were randomized into either a control (n = 5) or a hydrogen group (n = 5). After fibrillation by electric shock, no further treatment was administered in order to induce warm ischaemic injury for 1 h. The lungs were then procured, followed by 4 h of EVLP. During EVLP, the lungs were ventilated with room air in the control group, and with 2% hydrogen gas in the hydrogen group. Oxygen capacity (OC), pulmonary vascular resistance (PVR) and peak airway pressure (PAP) were measured every hour, and the expressions of interleukin-1 beta (IL-1β), IL-6 (IL-6), IL-8 (IL-8) and tumour necrosis factor-alpha (TNF-α) were evaluated in lung tissue after EVLP. Pathological evaluations were performed using lung injury severity (LIS) scores and the wet/dry ratio was also measured. RESULTS: The OC in the hydrogen group was higher than in the control group, but the difference was not statistically significant (P = 0.0862). PVR (P = 0.0111) and PAP (P = 0.0189) were statistically significantly lower in the hydrogen group. Compared with the control group, the hydrogen group had a statistically significantly lower expression of IL-1β (P = 0.0317), IL-6 (P = 0.0159), IL-8 (P = 0.0195) and TNF-α (P = 0.0159). The LIS scores (P = 0.0358) and wet/dry ratios (P = 0.040) were also significantly lower in the hydrogen group. CONCLUSIONS: Hydrogen gas inhalation during EVLP improved the function of DCD lungs, which may increase the utilization of DCD lungs.
Full Text
http://ejcts.oxfordjournals.org/content/48/4/542.long
DOI
10.1093/ejcts/ezv057
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers
Yonsei Authors
박무석(Park, Moo Suk) ORCID logo https://orcid.org/0000-0003-0820-7615
백효채(Paik, Hyo Chae) ORCID logo https://orcid.org/0000-0001-9309-8235
송주한(Song, Joo Han)
이성수(Lee, Sung Soo) ORCID logo https://orcid.org/0000-0001-8998-9510
임범진(Lim, Beom Jin) ORCID logo https://orcid.org/0000-0003-2856-0133
함석진(Haam, Seok Jin)
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/139908
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