0 205

Cited 25 times in

Characterization of adipose tissue-derived stromal vascular fraction for clinical application to cartilage regeneration

 Yeonsue Jang  ;  Yong Gon Koh  ;  Yun-Jin Choi  ;  Sung-Hwan Kim  ;  Dong Suk Yoon  ;  Moses Lee  ;  Jin Woo Lee 
 In Vitro Cellular & Developmental Biology-Animal, Vol.51(2) : 142-150, 2015 
Journal Title
 In Vitro Cellular & Developmental Biology-Animal 
Issue Date
Bone marrow concentration (BMC) is the most recognized procedure to prepare mesenchymal stem cells for cartilage regeneration. However, bone marrow aspiration is highly invasive and results in low stem cell numbers. Recently, adipose tissue-derived stromal vascular fraction (AT-SVF) was studied as an alternate source of stem cells for cartilage regeneration. However, AT-SVF is not fully characterized in terms of functional equivalence to BMC. Therefore, in this study, we characterized AT-SVF and assessed its suitability as a one-step surgical procedure for cartilage regeneration, as an alternative to BMC. AT-SVF contained approximately sixfold less nucleated cells than BMC. However, adherent cells in AT-SVF were fourfold greater than BMC. Additionally, the colony-forming unit frequency of AT-SVF was higher than that of BMC, at 0.5 and 0.01%, respectively. The mesenchymal stem cell (MSC) population (CD45-CD31-CD90+CD105+) was 4.28% in AT-SVF and 0.42% in BMC, and the adipose-derived stromal cell (ASC) population (CD34+CD31-CD146-) was 32% in AT-SVF and 0.16% in BMC. In vitro chondrogenesis demonstrated that micromass was not formed in BMC, whereas it was clearly formed in AT-SVF. Taken together, uncultured AT-SVF could be used in one-step surgery for cartilage regeneration as a substitute for BMC.
Full Text
Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sung Hwan(김성환) ORCID logo https://orcid.org/0000-0001-5743-6241
Yoon, Dong Suk(윤동석)
Lee, Mo Ses(이모세)
Lee, Jin Woo(이진우) ORCID logo https://orcid.org/0000-0002-0293-9017
Jang, Yeon Sue(장연수) ORCID logo https://orcid.org/0000-0001-5683-0001
RIS (EndNote)
XLS (Excel)
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.