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Protection of mice against pandemic H1N1 influenza virus challenge after immunization with baculovirus-expressed stabilizing peptide fusion hemagglutinin protein

DC Field Value Language
dc.contributor.author김종선-
dc.contributor.author박필구-
dc.contributor.author이재면-
dc.contributor.author조용근-
dc.date.accessioned2016-02-04T11:09:48Z-
dc.date.available2016-02-04T11:09:48Z-
dc.date.issued2015-
dc.identifier.issn1017-7825-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/139807-
dc.description.abstractCurrent influenza vaccines are produced in embryonated chicken eggs. However, egg-based vaccines have various problems. To address these problems, recombinant protein vaccines have been developed as new vaccine candidates. Unfortunately, recombinant proteins frequently encounter aggregation and low stability during their biogenesis. It has been previously demonstrated that recombinantly expressed proteins can be greatly stabilized with high solubility by fusing stabilizing peptide (SP) derived from the C-terminal acidic tail of human synuclein (ATS). To investigate whether SP fusion proteins can induce protective immunity in mice, we produced influenza HA and SP fusion protein using a baculovirus expression system. In in vitro tests, SP-fused recombinant HA1 (SP-rHA1) was shown to be more stable than recombinant HA1 (rHA1). Mice were immunized intramuscularly with baculovirus-expressed rHA1 protein or SP-rHA1 protein ($2{\mu}g/mouse$ 수식 이미지) formulated with aluminum hydroxide. Antibody responses were determined by ELISA and hemagglutination inhibition assay. We observed that SP-rHA1 immunization elicited HA-specific antibody responses that were comparable to rHA1 immunization. These results indicate that fusion of SP to rHA1 does not negatively affect the immunogenicity of the vaccine candidate. Therefore, it is possible to apply SP fusion technology to develop stable recombinant protein vaccines with high solubility.-
dc.description.statementOfResponsibilityopen-
dc.format.extent280~287-
dc.relation.isPartOfJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHAntibodies, Neutralizing/blood-
dc.subject.MESHAntibodies, Neutralizing/immunology-
dc.subject.MESHAntibodies, Viral/blood-
dc.subject.MESHAntibodies, Viral/immunology*-
dc.subject.MESHBaculoviridae/genetics*-
dc.subject.MESHDisease Models, Animal-
dc.subject.MESHHemagglutinin Glycoproteins, Influenza Virus/genetics-
dc.subject.MESHHemagglutinin Glycoproteins, Influenza Virus/immunology*-
dc.subject.MESHHumans-
dc.subject.MESHImmunity, Humoral-
dc.subject.MESHInfluenza A Virus, H1N1 Subtype/immunology*-
dc.subject.MESHInfluenza Vaccines/immunology*-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred BALB C-
dc.subject.MESHOrthomyxoviridae Infections/prevention & control*-
dc.subject.MESHRecombinant Fusion Proteins/immunology-
dc.subject.MESHSynucleins/genetics-
dc.subject.MESHVaccination-
dc.subject.MESHVaccines, Subunit/immunology-
dc.titleProtection of mice against pandemic H1N1 influenza virus challenge after immunization with baculovirus-expressed stabilizing peptide fusion hemagglutinin protein-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Microbiology (미생물학)-
dc.contributor.googleauthorEunji Yang-
dc.contributor.googleauthorYonggeun Cho-
dc.contributor.googleauthorJungah Choi-
dc.contributor.googleauthorYoung Joo Choi-
dc.contributor.googleauthorPil Gu Park-
dc.contributor.googleauthorEunsun Park-
dc.contributor.googleauthorChoong Hwan Lee-
dc.contributor.googleauthorHyeja Lee-
dc.contributor.googleauthorJongsun Kim-
dc.contributor.googleauthorJae Myun Lee-
dc.contributor.googleauthorManki Song-
dc.identifier.doi10.4014/jmb.1410.10035-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00921-
dc.contributor.localIdA01726-
dc.contributor.localIdA03071-
dc.contributor.localIdA03864-
dc.relation.journalcodeJ01594-
dc.identifier.eissn1738-8872-
dc.identifier.pmid25394603-
dc.subject.keywordPandemic-
dc.subject.keywordinfluenza-
dc.subject.keywordhemagglutinin-
dc.subject.keywordstabilizing peptide-
dc.contributor.alternativeNameKim, Jong Sun-
dc.contributor.alternativeNamePark, Pil Gu-
dc.contributor.alternativeNameLee, Jae Myun-
dc.contributor.alternativeNameCho, Yong Geun-
dc.contributor.affiliatedAuthorKim, Jong Sun-
dc.contributor.affiliatedAuthorPark, Pil Gu-
dc.contributor.affiliatedAuthorLee, Jae Myun-
dc.contributor.affiliatedAuthorCho, Yong Geun-
dc.rights.accessRightsfree-
dc.citation.volume25-
dc.citation.number2-
dc.citation.startPage280-
dc.citation.endPage287-
dc.identifier.bibliographicCitationJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, Vol.25(2) : 280-287, 2015-
dc.identifier.rimsid46573-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers

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