Cited 10 times in
Transgenic mouse model expressing P53(R172H), luciferase, EGFP, and KRAS(G12D) in a single open reading frame for live imaging of tumor
DC Field | Value | Language |
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dc.contributor.author | 노원상 | - |
dc.contributor.author | 문혁 | - |
dc.contributor.author | 정숙인 | - |
dc.contributor.author | 조경주 | - |
dc.contributor.author | 주혜림 | - |
dc.contributor.author | 한광협 | - |
dc.date.accessioned | 2016-02-04T11:08:05Z | - |
dc.date.available | 2016-02-04T11:08:05Z | - |
dc.date.issued | 2015 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/139741 | - |
dc.description.abstract | Genetically engineered mouse cancer models allow tumors to be imaged in vivo via co-expression of a reporter gene with a tumor-initiating gene. However, differential transcriptional and translational regulation between the tumor-initiating gene and the reporter gene can result in inconsistency between the actual tumor size and the size indicated by the imaging assay. To overcome this limitation, we developed a transgenic mouse in which two oncogenes, encoding P53(R172H) and KRAS(G12D), are expressed together with two reporter genes, encoding enhanced green fluorescent protein (EGFP) and firefly luciferase, in a single open reading frame following Cre-mediated DNA excision. Systemic administration of adenovirus encoding Cre to these mice induced specific transgene expression in the liver. Repeated bioluminescence imaging of the mice revealed a continuous increase in the bioluminescent signal over time. A strong correlation was found between the bioluminescent signal and actual tumor size. Interestingly, all liver tumors induced by P53(R172H) and KRAS(G12D) in the model were hepatocellular adenomas. The mouse model was also used to trace cell proliferation in the epidermis via live fluorescence imaging. We anticipate that the transgenic mouse model will be useful for imaging tumor development in vivo and for investigating the oncogenic collaboration between P53(R172H) and KRAS(G12D). | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.relation.isPartOf | SCIENTIFIC REPORTS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Proliferation | - |
dc.subject.MESH | Disease Models, Animal | - |
dc.subject.MESH | Genes, Reporter | - |
dc.subject.MESH | Green Fluorescent Proteins/genetics | - |
dc.subject.MESH | Green Fluorescent Proteins/metabolism* | - |
dc.subject.MESH | Liver/metabolism | - |
dc.subject.MESH | Liver/pathology | - |
dc.subject.MESH | Liver Neoplasms/metabolism | - |
dc.subject.MESH | Liver Neoplasms/mortality | - |
dc.subject.MESH | Liver Neoplasms/pathology* | - |
dc.subject.MESH | Luciferases/genetics | - |
dc.subject.MESH | Luciferases/metabolism* | - |
dc.subject.MESH | Luminescent Measurements | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred C57BL | - |
dc.subject.MESH | Mice, Transgenic | - |
dc.subject.MESH | NIH 3T3 Cells | - |
dc.subject.MESH | Open Reading Frames/genetics | - |
dc.subject.MESH | Optical Imaging | - |
dc.subject.MESH | Proto-Oncogene Proteins p21(ras)/genetics | - |
dc.subject.MESH | Proto-Oncogene Proteins p21(ras)/metabolism* | - |
dc.subject.MESH | Real-Time Polymerase Chain Reaction | - |
dc.subject.MESH | Skin/metabolism | - |
dc.subject.MESH | Skin/pathology | - |
dc.subject.MESH | Survival Rate | - |
dc.subject.MESH | Tumor Suppressor Protein p53/genetics | - |
dc.subject.MESH | Tumor Suppressor Protein p53/metabolism* | - |
dc.title | Transgenic mouse model expressing P53(R172H), luciferase, EGFP, and KRAS(G12D) in a single open reading frame for live imaging of tumor | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Hye-Lim Ju | - |
dc.contributor.googleauthor | Diego F. Calvisi | - |
dc.contributor.googleauthor | Hyuk Moon | - |
dc.contributor.googleauthor | Sinhwa Baek | - |
dc.contributor.googleauthor | Silvia Ribback | - |
dc.contributor.googleauthor | Frank Dombrowski | - |
dc.contributor.googleauthor | Kyung Joo Cho | - |
dc.contributor.googleauthor | Sook In Chung | - |
dc.contributor.googleauthor | Kwang-Hyub Han | - |
dc.contributor.googleauthor | Simon Weonsang Ro | - |
dc.identifier.doi | 10.1038/srep08053 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01391 | - |
dc.contributor.localId | A03640 | - |
dc.contributor.localId | A03804 | - |
dc.contributor.localId | A03961 | - |
dc.contributor.localId | A04268 | - |
dc.contributor.localId | A01286 | - |
dc.relation.journalcode | J02646 | - |
dc.identifier.eissn | 2045-2322 | - |
dc.identifier.pmid | 25623590 | - |
dc.contributor.alternativeName | Ro, Simon Weonsang | - |
dc.contributor.alternativeName | Moon, Hyuk | - |
dc.contributor.alternativeName | Chung, Sook In | - |
dc.contributor.alternativeName | Cho, Kyuong Joo | - |
dc.contributor.alternativeName | Ju, Hye Lim | - |
dc.contributor.alternativeName | Han, Kwang Hyup | - |
dc.contributor.affiliatedAuthor | Moon, Hyuk | - |
dc.contributor.affiliatedAuthor | Chung, Sook In | - |
dc.contributor.affiliatedAuthor | Cho, Kyuong Joo | - |
dc.contributor.affiliatedAuthor | Ju, Hye Lim | - |
dc.contributor.affiliatedAuthor | Han, Kwang Hyup | - |
dc.contributor.affiliatedAuthor | Ro, Simon Weonsang | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 5 | - |
dc.citation.startPage | 8053 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, Vol.5 : 8053, 2015 | - |
dc.identifier.rimsid | 52988 | - |
dc.type.rims | ART | - |
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