507 479

Cited 28 times in

Local protein synthesis in neuronal axons: why and how we study

Authors
 Eunjin Kim  ;  Hosung Jung 
Citation
 BMB REPORTS, Vol.48(3) : 139-146, 2015 
Journal Title
BMB REPORTS
ISSN
 1976-6696 
Issue Date
2015
MeSH
Axons* ; Nerve Tissue Proteins/biosynthesis* ; Neurons/metabolism*
Keywords
Axon ; Axon-TRAP ; BONCAT ; Boyden chamber ; Campenot chamber ; Compartmentalized culture ; Laser capture microdissection ; Local mRNA translation ; Microfluidic device ; Neuron ; Protein synthesis ; Puromycin tagging ; Ribosome ; SILAC
Abstract
Adaptive brain function and synaptic plasticity rely on dynamic regulation of local proteome. One way for the neuron to introduce new proteins to the axon terminal is to transport those from the cell body, which had long been thought as the only source of axonal proteins. Another way, which is the topic of this review, is synthesizing proteins on site by local mRNA translation. Recent evidence indicates that the axon stores a reservoir of translationally silent mRNAs and regulates their expression solely by translational control. Different stimuli to axons, such as guidance cues, growth factors, and nerve injury, promote translation of selective mRNAs, a process required for the axon's ability to respond to these cues. One of the critical questions in the field of axonal protein synthesis is how mRNA-specific local translation is regulated by extracellular cues. Here, we review current experimental techniques that can be used to answer this question. Furthermore, we discuss how new technologies can help us understand what biological processes are regulated by axonal protein synthesis in vivo.
Files in This Item:
T201500781.pdf Download
DOI
10.5483/BMBRep.2015.48.3.010
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Jung, Ho Sung(정호성) ORCID logo https://orcid.org/0000-0002-5059-8050
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/139694
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links