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Role of VcrD1 protein in expression and secretion of flagellar components in Vibrio parahaemolyticus

 Hyeon Jin Noh  ;  Sara Nagami  ;  Min Jung Kim  ;  Juri Kim  ;  Na Kyung Lee  ;  Kyu-Ho Lee  ;  Soon-Jung Park 
 ARCHIVES OF MICROBIOLOGY, Vol.197(3) : 397-410, 2015 
Journal Title
Issue Date
Bacterial Proteins*/genetics ; Bacterial Proteins*/secretion ; Flagella/genetics* ; Flagella/secretion ; Gene Expression Regulation, Bacterial* ; Genetic Complementation Test ; Mutation ; Vibrio parahaemolyticus/genetics* ; Vibrio parahaemolyticus/metabolism*
Vibrio parahaemolyticus ; VcrD1 protein ; Flagellar proteins
VcrD1 protein is a component of type III secretion system (T3SS) 1 in Vibrio parahaemolyticus. A comparative analysis of secretomes of wild-type and ΔvcrD1 strains revealed that the mutant was defective in secretion of diverse proteins including several flagellar components. Western blot analyses using specific antibodies confirmed that the secretion of at least four flagellar components, such as FlaA, FlgL, FlgE, and FlgM, was affected by the vcrD1 mutation, which was consistent with decreased motility on soft agar plates and the non-flagellated morphology of the mutant. The ΔexsA mutant, another T3SS1 mutant, did not showed reduced motility, but became non-motile phenotype with the additional ΔvcrD1 mutation. Complementation of wild-type vcrD1 gene into ΔvcrD1 mutant resulted in restored motility. Fractionation of bacterial cytoplasm from the periplasm and membrane revealed lower levels of FlaA and FlgM in the cytoplasm of the ΔvcrD1 mutant, indicating that VcrD1 might regulate the expression of flagellar genes in addition to the secretion of flagellar components in V. parahaemolyticus. Quantitative RT-PCR assays of seven representative flagellar genes in the wild-type and ΔvcrD1 mutant strains demonstrated that transcript levels of two early flagellar genes, flaK and flaL, were not reduced by the vcrD1 mutation, whereas the middle and late flagellar genes were expressed at a lower level in the vcrD1 mutant. This study raises a possibility that VcrD1 plays a role in flagellar morphogenesis in V. parahaemolyticus by regulating the expression and secretion of flagellar components.
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1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Min Jung(김민정)
Kim, Ju Ri(김주리) ORCID logo https://orcid.org/0000-0001-8270-7584
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
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