Cited 9 times in
microDuMIP: target-enrichment technique for microarray-based duplex molecular inversion probes
DC Field | Value | Language |
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dc.contributor.author | 김한상 | - |
dc.contributor.author | 이민구 | - |
dc.contributor.author | 이지현 | - |
dc.contributor.author | 한수민 | - |
dc.date.accessioned | 2016-02-04T11:05:12Z | - |
dc.date.available | 2016-02-04T11:05:12Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/139632 | - |
dc.description.abstract | Molecular inversion probe (MIP)-based capture is a scalable and effective target-enrichment technology that can use synthetic single-stranded oligonucleotides as probes. Unlike the straightforward use of synthetic oligonucleotides for low-throughput target capture, high-throughput MIP capture has required laborious protocols to generate thousands of single-stranded probes from DNA microarray because of multiple enzymatic steps, gel purifications and extensive PCR amplifications. Here, we developed a simple and efficient microarray-based MIP preparation protocol using only one enzyme with double-stranded probes and improved target capture yields by designing probes with overlapping targets and unique barcodes. To test our strategy, we produced 11 510 microarray-based duplex MIPs (microDuMIPs) and captured 3554 exons of 228 genes in a HapMap genomic DNA sample (NA12878). Under our protocol, capture performance and precision of calling were compatible to conventional MIP capture methods, yet overlapping targets and unique barcodes allowed us to precisely genotype with as little as 50 ng of input genomic DNA without library preparation. microDuMIP method is simpler and cheaper, allowing broader applications and accurate target sequencing with a scalable number of targets. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | e28~e28 | - |
dc.relation.isPartOf | NUCLEIC ACIDS RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | DNA, Single-Stranded/genetics* | - |
dc.subject.MESH | Exome/genetics | - |
dc.subject.MESH | Molecular Probe Techniques* | - |
dc.subject.MESH | Oligonucleotide Array Sequence Analysis/methods* | - |
dc.subject.MESH | Oligonucleotide Probes/genetics* | - |
dc.subject.MESH | Polymerase Chain Reaction | - |
dc.subject.MESH | Polymorphism, Single Nucleotide | - |
dc.subject.MESH | Reproducibility of Results | - |
dc.subject.MESH | Sequence Analysis, DNA/methods | - |
dc.title | microDuMIP: target-enrichment technique for microarray-based duplex molecular inversion probes | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학) | - |
dc.contributor.googleauthor | Jung-Ki Yoon | - |
dc.contributor.googleauthor | Jinwoo Ahn | - |
dc.contributor.googleauthor | Han Sang Kim | - |
dc.contributor.googleauthor | Soo Min Han | - |
dc.contributor.googleauthor | Hoon Jang | - |
dc.contributor.googleauthor | Min Goo Lee | - |
dc.contributor.googleauthor | Ji Hyun Lee | - |
dc.contributor.googleauthor | Duhee Bang | - |
dc.identifier.doi | 10.1093/nar/gku1188 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02781 | - |
dc.contributor.localId | A04292 | - |
dc.contributor.localId | A03214 | - |
dc.contributor.localId | A01098 | - |
dc.relation.journalcode | J02387 | - |
dc.identifier.eissn | 1362-4962 | - |
dc.identifier.pmid | 25414325 | - |
dc.contributor.alternativeName | Kim, Han Sang | - |
dc.contributor.alternativeName | Lee, Min Goo | - |
dc.contributor.alternativeName | Lee, Ji Hyun | - |
dc.contributor.alternativeName | Han, Soo Min | - |
dc.contributor.affiliatedAuthor | Lee, Min Goo | - |
dc.contributor.affiliatedAuthor | Han, Soo Min | - |
dc.contributor.affiliatedAuthor | Lee, Ji Hyun | - |
dc.contributor.affiliatedAuthor | Kim, Han Sang | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 43 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 28 | - |
dc.identifier.bibliographicCitation | NUCLEIC ACIDS RESEARCH, Vol.43(5) : 28, 2015 | - |
dc.identifier.rimsid | 52371 | - |
dc.type.rims | ART | - |
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