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The sul1 gene in Stenotrophomonas maltophilia with high-level resistance to trimethoprim/sulfamethoxazole

Authors
 Hae-Sun Chung  ;  Kyeongmi Kim  ;  Sang Sook Hong  ;  Seong Geun Hong  ;  Kyungwon Lee  ;  Yunsop Chong 
Citation
 ANNALS OF LABORATORY MEDICINE, Vol.35(2) : 246-249, 2015 
Journal Title
 ANNALS OF LABORATORY MEDICINE 
ISSN
 2234-3806 
Issue Date
2015
MeSH
Anti-Bacterial Agents/pharmacology ; Bacterial Proteins/genetics* ; Drug Resistance, Bacterial/genetics ; Gram-Negative Bacterial Infections/microbiology ; Gram-Negative Bacterial Infections/pathology ; Humans ; Integrons/genetics* ; Microbial Sensitivity Tests ; Stenotrophomonas maltophilia/drug effects* ; Stenotrophomonas maltophilia/genetics ; Stenotrophomonas maltophilia/isolation & purification ; Trimethoprim, Sulfamethoxazole Drug Combination/pharmacology*
Keywords
Class 1 integron ; Stenotrophomonas maltophilia ; Trimethoprim/sulfamethoxazole ; sul
Abstract
Emerging resistance to trimethoprim/sulfamethoxazole (SXT) poses a serious threat to the treatment of Stenotrophomonas maltophilia infections. We determined the prevalence and molecular characteristics of acquired SXT resistance in recent clinical S. maltophilia isolates obtained from Korea. A total of 252 clinical isolates of S. maltophilia were collected from 10 university hospitals in Korea between 2009 and 2010. Antimicrobial susceptibility was determined by using the CLSI agar dilution method. The sul1, sul2, and sul3 genes, integrons, insertion sequence common region (ISCR) elements, and dfrA genes were detected using PCR. The presence of the sul1 gene and integrons was confirmed through sequence analysis. Among the 32 SXT-resistant isolates, sul1 was detected in 23 isolates (72%), all of which demonstrated high-level resistance (≥64 mg/L) to SXT. The sul1 gene (varying in size and structure) was linked to class 1 integrons in 15 of the 23 isolates (65%) harboring this gene. None of the SXT-susceptible isolates or the SXT-resistant isolates with a minimum inhibitory concentration of 4 and 8 mg/L were positive for sul1. Moreover, the sul2, sul3, and dfrA genes or the ISCR elements were not detected. The sul1 gene may play an important role in the high-level SXT resistance observed in S. maltophilia.
Files in This Item:
T201500552.pdf Download
DOI
10.3343/alm.2015.35.2.246
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
Chong, Yun Sop(정윤섭)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/139542
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