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Discrimination between active and latent tuberculosis based on ratio of antigen-specific to mitogen-induced IP-10 production

DC Field Value Language
dc.contributor.author강영애-
dc.contributor.author신성재-
dc.contributor.author조상래-
dc.contributor.author허윤경-
dc.contributor.author이혜존-
dc.date.accessioned2016-02-04T11:00:48Z-
dc.date.available2016-02-04T11:00:48Z-
dc.date.issued2015-
dc.identifier.issn0095-1137-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/139472-
dc.description.abstractMycobacterium tuberculosis is the major causative agent of tuberculosis (TB). The gamma interferon (IFN-γ) release assay (IGRA) has been widely used to diagnose TB by testing cell-mediated immune responses but has no capacity for distinguishing between active TB and latent TB infection (LTBI). This study aims to identify a parameter that will help to discriminate active TB and LTBI. Whole-blood samples from 33 active TB patients, 20 individuals with LTBI, and 26 non-TB controls were applied to the commercial IFN-γ release assay, QuantiFERON-TB Gold In-Tube, and plasma samples were analyzed for interleukin-2 (IL-2), IL-6, IL-8, IL-10, IL-13, tumor necrosis factor-alpha (TNF-α), IFN-γ, monokine induced by IFN-γ (MIG), interferon gamma inducible protein 10 (IP-10), interferon-inducible T cell alpha chemoattractant (I-TAC), and monocyte chemoattractant protein 1 (MCP-1) by using a commercial cytometric bead array. The Mycobacterium tuberculosis antigen-specific production of most of the assayed cytokines and chemokines was higher in the active TB than in the LTBI group. The mitogen-induced responses were lower in the active TB than in the LTBI group. When the ratio of TB-specific to mitogen-induced responses was calculated, IL-2, IL-6, IL-10, IL-13, TNF-α, IFN-γ, MIG, and IP-10 were more useful in discriminating active TB from LTBI. In particular, most patients showed higher IP-10 production to Mycobacterium tuberculosis antigens than to mitogen at the individual level, and the ratio for IP-10 was the strongest indicator of active infection versus LTBI with 93.9% sensitivity and 90% specificity. In conclusion, the ratio of the TB-specific to the mitogen-induced IP-10 responses showed the most promising accuracy for discriminating active TB versus LTBI and should be further studied to determine whether it can serve as a biomarker that might help clinicians administer appropriate treatments.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.relation.isPartOfJOURNAL OF CLINICAL MICROBIOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdult-
dc.subject.MESHAntigens, Bacterial/immunology*-
dc.subject.MESHChemokine CXCL10/secretion*-
dc.subject.MESHDiagnosis, Differential-
dc.subject.MESHFemale-
dc.subject.MESHHumans-
dc.subject.MESHInterferon-gamma Release Tests/methods*-
dc.subject.MESHLeukocytes, Mononuclear/immunology*-
dc.subject.MESHMale-
dc.subject.MESHMiddle Aged-
dc.subject.MESHMitogens/metabolism*-
dc.subject.MESHMycobacterium tuberculosis/immunology*-
dc.subject.MESHTuberculosis/diagnosis*-
dc.subject.MESHYoung Adult-
dc.titleDiscrimination between active and latent tuberculosis based on ratio of antigen-specific to mitogen-induced IP-10 production-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorYun Hee Jeong-
dc.contributor.googleauthorYun-Gyoung Hur-
dc.contributor.googleauthorHyejon Lee-
dc.contributor.googleauthorSunghyun Kim-
dc.contributor.googleauthorJang-Eun Cho-
dc.contributor.googleauthorJun Chang-
dc.contributor.googleauthorSung Jae Shin-
dc.contributor.googleauthorHyeyoung Lee-
dc.contributor.googleauthorYoung Ae Kang-
dc.contributor.googleauthorSang-Nae Cho-
dc.contributor.googleauthorSang-Jun Ha-
dc.identifier.doi10.1128/JCM.02758-14-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02114-
dc.contributor.localIdA00057-
dc.contributor.localIdA03824-
dc.contributor.localIdA04701-
dc.relation.journalcodeJ01325-
dc.identifier.eissn1098-660X-
dc.identifier.pmid25428147-
dc.contributor.alternativeNameKang, Young Ae-
dc.contributor.alternativeNameShin, Sung Jae-
dc.contributor.alternativeNameCho, Sang Nae-
dc.contributor.alternativeNameHur, Yun Gyoung-
dc.contributor.affiliatedAuthorShin, Sung Jae-
dc.contributor.affiliatedAuthorKang, Young Ae-
dc.contributor.affiliatedAuthorCho, Sang Nae-
dc.contributor.affiliatedAuthorHur, Yun-Gyoung-
dc.rights.accessRightsfree-
dc.citation.volume53-
dc.citation.number2-
dc.citation.startPage504-
dc.citation.endPage510-
dc.identifier.bibliographicCitationJOURNAL OF CLINICAL MICROBIOLOGY, Vol.53(2) : 504-510, 2015-
dc.identifier.rimsid55416-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers

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