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Cytotoxicity and terminal differentiation of human oral keratinocyte by indium ions from a silver-palladium-gold-indium dental alloy

 Jung-Hwan Lee  ;  Sang-Hee Seo  ;  Sang-Bae Lee  ;  Ji-Yeon Om  ;  Kwang-Mahn Kim  ;  Kyoung-Nam Kim 
 DENTAL MATERIALS, Vol.31(2) : 123-133, 2015 
Journal Title
Issue Date
Blotting, Western ; Cell Differentiation/drug effects* ; Corrosion ; Dental Alloys/chemistry* ; Electrochemical Techniques ; Enzyme-Linked Immunosorbent Assay ; Fibronectins/metabolism ; Gold Alloys/chemistry ; Humans ; Indium/chemistry ; Ions ; Keratinocytes/drug effects* ; Keratinocytes/metabolism ; Keratins/metabolism ; Materials Testing ; Palladium/chemistry ; Protein Precursors/metabolism ; Reactive Oxygen Species/metabolism ; Receptor, Epidermal Growth Factor/metabolism ; Silver/chemistry ; Spectrophotometry, Atomic ; X-Ray Diffraction
Silver–palladium–gold–indium dental alloy ; Corrosion resistance ; Indium ions ; Cytotoxicity ; Terminal differentiation ; Human oral keratinocytes
OBJECTIVE: Dental alloys containing indium (In) have been used in dental restoration for two decades; however, no study has investigated the biological effects of In ions, which may be released in the oral cavity, on human oral keratinocytes. The objective of the present study was to investigate the biological effects of In ions on human oral keratinocyte after confirming their release from a silver-palladium-gold-indium (Ag-Pd-Au-In) dental alloy.

METHODS: As a corrosion assay, a static immersion tests were performed by detecting the released ions in the corrosion solution from the Ag-Pd-Au-In dental alloy using inductively coupled plasma atomic emission spectroscopy. The cytotoxicity and biological effects of In ions were then studied with In compounds in three human oral keratinocyte cell lines: immortalized human oral keratinocyte (IHOK), HSC-2, and SCC-15.

RESULTS: Higher concentrations of In and Cu ions were detected in Ag-Pd-Au-In (P<0.05) than in Ag-Pd-Au, and AgCl deposition occurred on the surface of Ag-Pd-Au-In after a 7-day corrosion test due to its low corrosion resistance. At high concentrations, In ions induced cytotoxicity; however, at low concentrations (∼0.8In(3+)mM), terminal differentiation was observed in human oral keratinocytes. Intracellular ROS was revealed to be a key component of In-induced terminal differentiation.

SIGNIFICANCE: In ions were released from dental alloys containing In, and high concentrations of In ions resulted in cytotoxicity, whereas low concentrations induced the terminal differentiation of human oral keratinocytes via increased intracellular ROS. Therefore, dental alloys containing In must be biologically evaluated for their safe use.
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2. College of Dentistry (치과대학) > Dept. of Dental Biomaterials and Bioengineering (치과생체재료공학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyoung Nam(김경남)
Kim, Kwang Mahn(김광만) ORCID logo https://orcid.org/0000-0002-5235-0294
Seo, Sang Hee(서상희)
Om, Ji Yeon(엄지연)
Lee, Jung Hwan(이정환)
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