Apatite-coated collagen sponge for the delivery of bone morphogenetic protein-2 in rabbit posterolateral lumbar fusion

Abstract

Bone morphogenetic proteins (BMPs) need an effective delivery system for efficient bone regeneration. In this study, we evaluated the efficiency of an apatite-coated collagen sponge for the long-term delivery of BMP-2 in a rabbit model of lumbar posterolateral fusion. A total of 15 rabbits, divided into three groups, underwent posterolateral lumbar fusion. The first group (control group) received uncoated collagen sponges without BMP-2. The second group (uncoated group) received uncoated collagen sponges with BMP-2 (40 μg each side). The third group (apatite-coated group) received apatite-coated collagen sponges with the same level of BMPs (40 μg each side). All rabbits were euthanized 6 weeks after operation, and the fusion status was assessed by radiographic study, micro-CT, manual palpation, biomechanical study, and histological examination. Fusion rates as determined by radiographic study, micro-CT, and manual palpation showed that the apatite-coated group had a significantly higher rate of fusion than the control group (P = 0.024), while the uncoated group did not (P = 0.083). Biomechanical study showed significantly higher tensile strength in the apatite-coated group than the uncoated group (P = 0.032). Denser trabeculations were found in the apatite-coated group compared with the uncoated group. It is concluded that the use of apatite-coated collagen sponges for BMP-2 delivery enhanced bone regeneration.