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Giardia lamblia binding immunoglobulin protein triggers maturation of dendritic cells via activation of TLR4-MyD88-p38 and ERK1/2 MAPKs.

 H Y Lee  ;  J Kim  ;  H J Noh  ;  H P Kim  ;  S J Park 
 PARASITE IMMUNOLOGY, Vol.36(12) : 627-646, 2014 
Journal Title
Issue Date
Animals ; Cytokines/immunology ; Dendritic Cells/cytology* ; Dendritic Cells/immunology* ; Female ; Giardia lamblia/physiology* ; Giardiasis/immunology* ; HSP70 Heat-Shock Proteins/metabolism* ; Immunoglobulins/metabolism* ; Inflammation/immunology ; Inflammation/metabolism ; MAP Kinase Signaling System ; Mice, Inbred BALB C ; NF-kappa B/metabolism ; Protozoan Proteins/metabolism* ; Signal Transduction ; T-Lymphocytes/metabolism ; Toll-Like Receptor 4/immunology ; Transcription Factor AP-1/metabolism
Giardia lamblia ; binding immunoglobulin protein ; dendritic cell maturation
Much remains unknown about the mammalian immune response to Giardia lamblia, a protozoan pathogen that causes diarrhoeal outbreaks. We fractionated protein extracts of G. lamblia trophozoites by Viva-spin centrifugation, DEAE ion exchange and gel filtration chromatography. Resultant fractions were screened for antigenic molecules by western blots analysis using anti-G. lamblia antibodies (Abs), resulting in identification of G. lamblia binding immunoglobulin protein (GlBiP). Maturation of mouse dendritic cells (DCs) in response to recombinant GlBiP (rGlBiP) was detected by increased expression of surface molecules such as CD80, CD86 and MHC class II; these mature DCs, produced pro-inflammatory cytokines (TNF-α, IL-12 and IL-6). Especially, the truncated rGlBiP containing the heat-shock protein 70 domain-induced cytokine production from mouse DCs. rGlBiP-induced DC activation was initiated by TLR4 in a MyD88-dependent way and occurred through activation of p38 and ERK1/2 MAPKs as well as increased activity of NF-κB and AP-1. Moreover, CD4(+) T cells stimulated with rGlBiP-treated DCs produced high levels of IL-2 and IFN-γ. Together, our results suggest that GlBiP contributes to maturation of DCs via activation of TLR4-MyD88-p38, ERK1/2 MAPK, NF-κB and AP-1.
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1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Ju Ri(김주리) ORCID logo https://orcid.org/0000-0001-8270-7584
Kim, Hyoung Pyo(김형표) ORCID logo https://orcid.org/0000-0003-1441-8822
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
Lee, Hye Yeon(이혜연)
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