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PLGA nanofiber membranes loaded with epigallocatechin-3-O-gallate are beneficial to prevention of postsurgical adhesions.

Authors
 Yong Cheol Shin  ;  Won Jun Yang  ;  Jong Ho Lee  ;  Jin Woo Oh  ;  Tai Wan Kim  ;  Jong Chul Park  ;  Suong Hyu Hyon  ;  Dong Wook Han 
Citation
 INTERNATIONAL JOURNAL OF NANOMEDICINE, Vol.9 : 4067-4078, 2014 
Journal Title
 INTERNATIONAL JOURNAL OF NANOMEDICINE 
ISSN
 1176-9114 
Issue Date
2014
MeSH
Animals ; Catechin/analogs & derivatives* ; Catechin/chemistry ; Catechin/pharmacokinetics ; Catechin/pharmacology ; Lactic Acid/chemistry* ; Lactic Acid/pharmacology ; Male ; Membranes, Artificial ; Nanofibers/chemistry* ; Partial Thromboplastin Time ; Peritoneum/drug effects ; Peritoneum/pathology ; Polyglycolic Acid/chemistry* ; Polyglycolic Acid/pharmacology ; Protective Agents/chemistry* ; Protective Agents/pharmacology* ; Rats ; Rats, Sprague-Dawley ; Tissue Adhesions/pathology ; Tissue Adhesions/prevention & control*
Keywords
antiadhesion ; epigallocatechin-3-O-gallate ; nanofiber membrane ; poly(lactic-co-glycolic acid) ; tissue-adhesion barrier
Abstract
This study concentrates on the development of biodegradable nanofiber membranes with controlled drug release to ensure reduced tissue adhesion and accelerated healing. Nanofibers of poly(lactic-co-glycolic acid) (PLGA) loaded with epigallocatechin-3-O-gallate (EGCG), the most bioactive polyphenolic compound in green tea, were electrospun. The physicochemical and biomechanical properties of EGCG-releasing PLGA (E-PLGA) nanofiber membranes were characterized by atomic force microscopy, EGCG release and degradation profiles, and tensile testing. In vitro antioxidant activity and hemocompatibility were evaluated by measuring scavenged reactive oxygen species levels and activated partial thromboplastin time, respectively. In vivo antiadhesion efficacy was examined on the rat peritonea with a surgical incision. The average fiber diameter of E-PLGA membranes was approximately 300-500 nm, which was almost similar to that of pure PLGA equivalents. E-PLGA membranes showed sustained EGCG release mediated by controlled diffusion and PLGA degradation over 28 days. EGCG did not adversely affect the tensile strength of PLGA membranes, whereas it significantly decreased the elastic modulus and increased the strain at break. E-PLGA membranes were significantly effective in both scavenging reactive oxygen species and extending activated partial thromboplastin time. Macroscopic observation after 1 week of surgical treatment revealed that the antiadhesion efficacy of E-PLGA nanofiber membranes was significantly superior to those of untreated controls and pure PLGA equivalents, which was comparable to that of a commercial tissue-adhesion barrier. In conclusion, the E-PLGA hybrid nanofiber can be exploited to craft strategies for the prevention of postsurgical adhesions.
Files in This Item:
T201405697.pdf Download
DOI
10.2147/IJN.S68197
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/138834
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